Objective

To give an overview and describe the strengths and weaknesses of immunoglobulin E (IgE) microarray and other multiplex assays that have been developed and are being used for allergy diagnostics.

Data Sources

Queries for IgE microarray and multiplex assays were conducted with PubMed and Google Scholar, searching for primary articles and review papers.

Study Selections

We focused on articles written in English on commercially available IgE multiplex assays that were reported in the allergy and immunology literature.

Results

Several commercial IgE assays that use microarray or other multiplex technology have been developed, and some have been implemented into clinical practice in Europe and Asia, with the Immuno Solid-Phase Allergen Chip being the most widely studied. Results of these assays generally correlate with results using “singleplex” IgE assays (eg, ImmunoCAP), though there can be variability among products and among allergens. A strength of the microarray technology is that IgE to a large number of allergens can be detected simultaneously in a single test, and only a small amount of patient serum is required. Cost, inadequate sensitivity under some scenarios, and difficulties with data interpretation, in some cases of 100 or more allergens, can be limitations.

Conclusion

IgE microarray assays are already a valuable tool in research applications. These assays, and also other forms of IgE multiplex assays, are likely to play an important role in the clinical practice of allergy in the future. Additional studies focused on clinical outcomes, and the development of more targeted allergen panels could facilitate increased clinical use.

中文翻译：

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微阵列和其他多重技术在过敏诊断中的应用

客观的

概述并描述免疫球蛋白 E (IgE) 微阵列和其他已开发并用于过敏诊断的多重检测的优势和劣势。

数据源

使用 PubMed 和 Google Scholar 对 IgE 微阵列和多重检测进行查询，搜索原始文章和评论论文。

研究选择

我们专注于用英文撰写的关于过敏和免疫学文献中报道的商用 IgE 多重检测的文章。

结果

已经开发了几种使用微阵列或其他多重技术的商业 IgE 测定，其中一些已在欧洲和亚洲实施到临床实践中，其中免疫固相过敏原芯片是研究最广泛的。这些测定的结果通常与使用“单一”IgE 测定（例如 ImmunoCAP）的结果相关，尽管产品和过敏原之间可能存在差异。微阵列技术的一个优点是可以在一次测试中同时检测到大量过敏原的IgE，并且只需要少量的患者血清。成本、某些情况下的敏感性不足以及数据解释的困难（在某些情况下是 100 种或更多过敏原）可能是限制因素。

结论

IgE 微阵列检测已经是研究应用中的一种有价值的工具。这些检测以及其他形式的 IgE 多重检测可能在未来的过敏临床实践中发挥重要作用。其他研究侧重于临床结果，开发更有针对性的过敏原面板可以促进临床使用的增加。