Generation of three iPSC cell lines (SIAISi006-A, SIAISi007-A and SIAISi008-A) from a 66-year-old Alzheimer’s disease (AD) patient and her two unaffected children from Chinese Han population,Stem Cell Research

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Generation of three iPSC cell lines (SIAISi006-A, SIAISi007-A and SIAISi008-A) from a 66-year-old Alzheimer’s disease (AD) patient and her two unaffected children from Chinese Han population
Stem Cell Research ( IF 0.8 ) Pub Date : 2021-01-12 , DOI: 10.1016/j.scr.2021.102172
Wenxin Zhang ₁ , Qiuting Dai ₁ , Yishi Hua ₂ , Weihao Di ₁ , Jinghui Guo ₁ , Jian Zhao ₁ , Yulei Deng ₃ , Ying Wang ₂

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A 66-year-old Chinese Han Alzheimer's Disease (AD) female patient and her two unaffected children donated their Peripheral blood mononuclear cells (PBMC). Non-integrating episomal vector system were used to reprogram their PBMCs with human OKSM (OCT3/4, KLF4, SOX2, and c-MYC) transcription factors. Immunocytochemistry for pluripotency makers confirmed the pluripotency of transgene-free iPSCs. Pluripotency was confirmed by the ability of iPSCs to spontaneously differentiate three germ layers in vitro as well. The iPSC line displayed a normal karyotype. This model provides insight into further pathological studies to research identify early biomarkers, study disease pedigrees, and also for drug testing purposes.

更新日期：2021-01-18

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