Thermal hydrolysis is a common pre-treatment, used before anaerobic digestion processes, to enhance the hydrolysis rate. However, formation of inhibitory compounds and the increase of liquid fraction colour have been identified as potential drawbacks. This study was oriented to study the methane production from simple substrates, subjected to thermal hydrolysis. A mixture of glycine and glucose at different concentrations was prepared, at a ratio similar to proteins and carbohydrates found in activated sludge. Two temperatures were tested. At 120 °C a decrease on biogas production rate was observed. On the other hand, at 165 °C generation of recalcitrant material was observed, causing a decrease in methane potential and COD degradation, when a mixture of glycine and glucose was used as substrate. This was atributed to the formation of recalcitrant compounds via Maillard reaction, hyphothesis supported by FTIR-ATR, which indicated the formation of amide II Bonds.

热水解是一种常见的预处理，用于厌氧消化之前，以提高水解速率。然而，已经发现抑制性化合物的形成和液体部分颜色的增加是潜在的缺点。这项研究的目的是研究从简单的底物进行热水解产生的甲烷。制备了不同浓度的甘氨酸和葡萄糖的混合物，其比例类似于活性污泥中发现的蛋白质和碳水化合物。测试了两个温度。在120°C时，观察到沼气生产率下降。另一方面，当使用甘氨酸和葡萄糖的混合物作为底物时，在165°C时观察到产生顽固性物质，导致甲烷电位降低和COD降解。