Gallbladder cancer (GBC) with poor prognosis has been a major cause of cancer-related deaths worldwide. In this study, we aimed to screen and identify crucial genes in GBC through integrative analysis of multiple datasets and further experimental validation. A candidate crucial gene, up-regulated haptoglobin (HP), was firstly screened, and then further analysis and validation mainly focused on whether higher enrichment level of HP was responsible for pathophysiological process of GBC. HP was found with diverse expression patterns in various cancer types, and the dynamic expression patterns indicated its spatiotemporal characteristics in different tissues and disease stages, implicating its role in multiple biological processes. Further experimental validation showed that HP could promote the GBC-SD cell proliferation, migration and invasion, implying its role in pathophysiological process of GBC. HP may have a crucial role in occurrence and development of GBC, and it provides possibility as a potential biomarker or target in cancer prognosis and treatment.

预后不良的胆囊癌（GBC）已成为全球范围内与癌症相关的死亡的主要原因。在这项研究中，我们旨在通过对多个数据集进行综合分析并进一步进行实验验证来筛选和鉴定GBC中的关键基因。首先筛选候选的关键基因，即触珠蛋白（HP）的上调，然后进一步的分析和验证主要集中在HP的较高富集水平是否与GBC的病理生理过程有关。发现HP在各种癌症类型中具有多种表达模式，动态表达模式表明其在不同组织和疾病阶段的时空特征，暗示其在多种生物学过程中的作用。进一步的实验验证表明，HP可以促进GBC-SD细胞的增殖，迁移和侵袭，暗示其在GBC病理生理过程中的作用。HP在GBC的发生和发展中可能起着至关重要的作用，它为癌症的预后和治疗提供了可能的潜在生物标志物或靶标。