Oleuropein is an important natural compound with many biological activities. How to get high purity oleuropein is an urgent issue to be solved. This study provided a method for separating and purifying high purity oleuropein from olive leaves using boric acid affinity resin and a novel solvent system. And the experimental kinetic and adsorption isotherm data of boric acid affinity resin were well–fitted pseudo–first–order kinetic model and Langmuir isotherm model, respectively. The dynamic desorption experiments showed that ethanol was the most efficient eluent while had the lowest oleuropein purity. However, after the novel solvent system treated only one cycle of adsorption and desorption, the purity of oleuropein increased by 5.55 folds from 12.31 ± 0.42% – 68.30 ± 2.08 %. The results can guide the separation and purification of high purity catechol natural products using boric acid affinity resin and mixed solvent systems.

橄榄苦苷是具有许多生物活性的重要的天然化合物。如何获得高纯度橄榄苦苷是一个亟待解决的问题。这项研究提供了一种使用硼酸亲和树脂和新型溶剂系统从橄榄叶中分离纯化高纯度橄榄苦苷的方法。硼酸亲和树脂的实验动力学和吸附等温线数据分别是拟合好的拟一阶动力学模型和Langmuir等温线模型。动态解吸实验表明乙醇是最有效的洗脱液，而橄榄苦苷纯度最低。但是，在新型溶剂系统仅处理了一个吸附和解吸循环之后，橄榄苦苷的纯度从12.31±0.42％– 68.30±2.08％增加了5.55倍。