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Mechanism of contaminant removal by algae-bacteria symbiosis in a PBR system during the treatment of anaerobic digestion effluents
Agricultural Water Management ( IF 5.9 ) Pub Date : 2021-01-12 , DOI: 10.1016/j.agwat.2020.106556
Lei Feng , Jianan Song , Huisong Gu , Xiaofei Zhen

Large volumes of anaerobic digestion effluents (ADEs) are generated by intense livestock and poultry farm activities. If untreated, these effluents represent a threat to the environment. Previous data have indicated that microalgae are able to grow in photobioreactors (PBR) where high concentrations of inorganic salts are present. In the present research, algae-assisted sequencing batch photo-bio-reactor (A-SBPBR) with single micro-algae systems and single activated sludge systems were developed. We studied the effects of the treatments on different parameters including COD, TN, AN, and TP. We analyzed changes in bacterial community diversity using the high-throughput sequencing analysis. The degradation mechanism of the ADEs was studied by means of algal-bacterial symbiosis. Experimental results indicated that contaminants were efficiently removed from the ADEs when using the A-SBPBR. After treatment, the degradation rates of COD, TN, AN, and TP were 73.78%, 80.67%, 89.74%, and 95.39%, respectively. The outlet concentrations of COD, TN, AN, and TP were 355.09 ± 17.90, 83.97 ± 9.37, 35.42 ± 2.65 and 0.87 ± 29 mg/L, respectively. In the initial stage of the process (day 1), the most abundant bacteria present in the ABS (algal-bacterial symbiosis system) included proteobacteria, bacteroidetes, actinobacteria, acidobacteria and chloroflexi. Among these, those with the highest abundance values were proteobacteria and bacteroidetes. Abundance values were 24.33% and 19.97%, respectively. A-SBPBR contained various microorganisms including aerobic bacteria, fungus, and chlorella. In a reciprocal interaction, bacteria and microalgae were able to use each other´s metabolites. Under intermittent aeration conditions, organic C, N, and P present in the macromolecules were converted to smaller species. C was transformed to CO2 and organic C. In addition, N and P stayed as part of small molecules that were used by aerobic bacteria and fungus. When ABS operation reached stability, hydrolytic acidifying bacteria, which belonged to acidobacteria, were able to degrade different organic compounds including saccharides.



中文翻译:

PBR系统中厌氧消化废水处理过程中藻类-细菌共生去除污染物的机理

大量的畜禽养殖场活动产生大量的厌氧消化废水(ADEs)。如果不加以处理,这些废水将对环境构成威胁。先前的数据表明,微藻能够在存在高浓度无机盐的光生物反应器(PBR)中生长。在本研究中,开发了具有单个微藻类系统和单个活性污泥系统的藻类辅助测序批处理光生物反应器(A-SBPBR)。我们研究了处理对不同参数(包括COD,TN,AN和TP)的影响。我们使用高通量测序分析了细菌群落多样性的变化。通过藻-细菌共生研究了ADEs的降解机理。实验结果表明,使用A-SBPBR可以有效地从ADE中去除污染物。处理后,COD,TN,AN和TP的降解率分别为73.78%,80.67%,89.74%和95.39%。COD,TN,AN和TP的出口浓度分别为355.09±17.90、83.97±9.37、35.42±2.65和0.87±29 mg / L。在该过程的初始阶段(第1天),ABS(藻-细菌共生系统)中存在的最丰富的细菌包括变形杆菌,拟杆菌,放线菌,酸性细菌和绿弯曲菌。其中,丰度值最高的是变形杆菌和类杆菌。丰度分别为24.33%和19.97%。A-SBPBR包含多种微生物,包括好氧细菌,真菌和小球藻。在互惠互动中,细菌和微藻能够利用彼此的代谢产物。在间歇曝气条件下,大分子中存在的有机碳,氮和磷被转化为较小的物种。C转化为CO2和有机C。此外,N和P仍然是需氧细菌和真菌使用的小分子的一部分。当ABS操作达到稳定时,属于酸性细菌的水解酸化细菌能够降解包括糖在内的各种有机化合物。

更新日期:2021-01-12
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