Amphiphilic diblock copolymers may assemble in aqueous solutions to form vesicles delimited by a polymeric double layer, also known as polymersomes, considered a more robust option to liposomes. Diblock copolymers may respond to pH, temperature, and other conditions. Because of such properties, polymersomes are currently being studied as drug delivery systems or as nanoreactors. pH-responsive polymersomes are potentially crucial because unusual pH gradients are present in cells under several physiological and pathological conditions. We synthesized two diblock copolymers of poly [2-(dimethylamino) ethyl methacrylate]-block-polystyrene (PDMAEMA-b-PS) via RAFT. We both developed new materials and better-understood polymersomes' properties with pH and temperature-responsive groups with these polymers. GPC, 1H-NMR, and FTIR characterized copolymers. The ionization equilibrium of the PDMAEMA amino groups on the polymersomes was analyzed by potentiometric titration and Zeta potential measurements. The hydrodynamic radius of the polymersomes in different pH and temperatures was analyzed by DLS. Entrapment of an electron paramagnetic resonance probe indicated the presence of a hydrophilic inner core. Negative staining transmission electron microscopy showed spherical aggregates and confirmed the diameter around 80 nm. These polymersomes with dual stimulus–response (i.e., pH and temperature) may be a platform for gene delivery and nanoreactors.

中文翻译：

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聚[2-（二甲氨基）甲基丙烯酸乙酯]-b-聚苯乙烯的刺激响应性聚合物囊泡

两亲性二嵌段共聚物可以在水溶液中组装形成由聚合物双层界定的囊泡，也称为聚合物囊泡，被认为是脂质体的更强大的选择。二嵌段共聚物可能会响应 pH、温度和其他条件。由于这些特性，目前正在研究聚合物囊泡作为药物递送系统或纳米反应器。pH 响应性聚合物囊泡可能是至关重要的，因为在几种生理和病理条件下，细胞中存在不寻常的 pH 梯度。我们通过 RAFT 合成了聚 [2-(二甲氨基) 甲基丙烯酸乙酯]-嵌段聚苯乙烯 (PDMAEMA-b-PS) 的两种二嵌段共聚物。我们都开发了新材料，并更好地理解了聚合物囊泡的特性，这些聚合物具有这些聚合物的 pH 和温度响应基团。GPC、1H-NMR、和 FTIR 表征的共聚物。聚合物囊泡上 PDMAEMA 氨基的电离平衡通过电位滴定和 Zeta 电位测量进行分析。通过 DLS 分析了聚合物囊泡在不同 pH 和温度下的流体动力学半径。电子顺磁共振探针的捕获表明存在亲水内核。负染色透射电子显微镜显示球形聚集体并确认直径约为 80 nm。这些具有双重刺激-反应（即 pH 和温度）的聚合物囊泡可能是基因传递和纳米反应器的平台。通过 DLS 分析了聚合物囊泡在不同 pH 和温度下的流体动力学半径。电子顺磁共振探针的捕获表明存在亲水内核。负染色透射电子显微镜显示球形聚集体并确认直径约为 80 nm。这些具有双重刺激-反应（即 pH 和温度）的聚合物囊泡可能是基因传递和纳米反应器的平台。通过 DLS 分析了聚合物囊泡在不同 pH 和温度下的流体动力学半径。电子顺磁共振探针的捕获表明存在亲水内核。负染色透射电子显微镜显示球形聚集体并确认直径约为 80 nm。这些具有双重刺激-反应（即 pH 和温度）的聚合物囊泡可能是基因传递和纳米反应器的平台。