The Arabidopsis RRTF1 promoter is transiently activated by salt stress over 6 hours, before it quickly reverts to pre-stress levels, even if the salt stress continues. This allows the short-term expression of components that, although initially beneficial to the plant, will have negative effects if continuously produced at high levels. We therefore tested the application of the RRTF1 promoter to drive the activity of the SHO gene which is responsible for the production of certain cytokinin derivatives in plants. Transgenic plants with RRTF1 promoter-controlled SHO activity showed significant improvements in seed germination and survival rates under salt stress without any phenotypic alterations. In addition, the transgenic seedlings displayed significantly improved recovery rates from salt stress compared with the wild type. These observations support the use of the RRTF1 promoter for controlled cytokinin production alleviating the negative impact of salt stress without affecting plant morphology.

拟南芥 RRTF1启动子会在6小时内被盐胁迫短暂激活，即使盐胁迫持续存在，该启动子也会迅速恢复到预应力水平。这允许成分的短期表达，尽管这些成分最初对植物有益，但如果连续高水平生产，将产生负面影响。因此，我们测试了RRTF1启动子在驱动SHO基因活性中的应用，该基因负责植物中某些细胞分裂素衍生物的产生。具有RRTF1启动子控制的SHO的转基因植物在没有盐分胁迫的情况下，盐分胁迫的活性显示出种子发芽和存活率的显着提高。另外，与野生型相比，转基因幼苗显示出从盐胁迫下的回收率显着提高。这些观察结果支持使用RRTF1启动子来控制细胞分裂素的产生，从而减轻盐胁迫的负面影响而又不影响植物形态。