Inflammatory reactions in the body have been shown to contribute to migraine development. Therefore, genes involved in the inflammatory pathways might play a role in the susceptibility and development of migraine. In this study, polymorphisms in tumor necrosis factor alpha (TNFα) and lymphotoxin alpha (LTA) genes were tested for association with migraine. A total of 398 participants (198 migraine patients and 200 controls) were recruited in the study. Serum TNF level was measured using a sandwich ELISA kit. Lymphocytes’ and monocytes’ counts were obtained from a differential complete blood count profile. Participants’ DNA was extracted and genotyped for rs1800629 and rs1799724 in TNFα, and rs909253 in LTA. Controls had a significantly higher mean lymphocyte count (P = 0.018), while the mean monocyte count and serum TNFα levels did not differ between the two groups (P > 0.05). With respect to gene polymorphisms, the rs1800629 and rs1799724 variants showed significant association with migraine in all subjects, and in males and females when analyzed separately (P < 0.001). The rs909253 did not show any statistical difference in frequencies among the two groups (P > 0.05). Having the A allele in rs1800629 was associated with a higher risk of migraine in both male (OR, 95%; CI, G/A = 3.79 [1.87-7.69]; A/A = 14.22 [1.67-121.14]; P < 0.01) and female (OR, 95%; G/A = 2.54 [1.47-4.38]; A/A = 2.52 [1.12-5.69]; P < 0.001) subjects. In conclusion, rs1800629 and rs1799724 in TNFα showed significant association with migraine among the Jordanian population.

体内的炎症反应已被证明有助于偏头痛的发展。因此，参与炎症通路的基因可能在偏头痛的易感性和发展中发挥作用。在这项研究中，检测了肿瘤坏死因子α ( TNFα ) 和淋巴毒素α ( LTA ) 基因的多态性与偏头痛的关联。该研究共招募了 398 名参与者（198 名偏头痛患者和 200 名对照）。使用夹心ELISA试剂盒测量血清TNF水平。淋巴细胞和单核细胞的计数是从不同的全血细胞计数谱中获得的。提取参与者的 DNA 并对TNFα中的 rs1800629 和 rs1799724以及LTA中的 rs909253 进行基因分型. 对照组的平均淋巴细胞计数显着升高（P = 0.018），而两组的平均单核细胞计数和血清TNFα水平没有差异（P > 0.05）。在基因多态性方面，rs1800629 和 rs1799724 变异在所有受试者中显示出与偏头痛显着相关，在单独分析时在男性和女性中均显示出显着相关性（P < 0.001）。rs909253在两组之间的频率差异无统计学意义（P > 0.05）。rs1800629 中的 A 等位基因与男性偏头痛的较高风险相关（OR，95%；CI，G/A = 3.79 [1.87-7.69]；A/A = 14.22 [1.67-121.14]；P < 0.01 ) 和女性 (OR, 95%; G/A = 2.54 [1.47-4.38]; A/A = 2.52 [1.12-5.69];P < 0.001) 科目。总之，TNFα中的 rs1800629 和 rs1799724与约旦人群的偏头痛显着相关。