Plant height (PH) is closely associated with yield-related traits and environmental adaptation. Seven pairs of near-isogenic lines (NILs) targeting four QTL on 3AL, 4BL, 4AS, and 7AL wheat chromosome arms were assessed for PH and four yield-related traits including yield per plant (Y/P), grain number per spike (G/S), thousand kernel weight (TKW), and biomass per plant (B/P). Significant differences were observed in the NIL pairs for the measured traits. NIL pairs targeting the 3AL QTL differed significantly in PH, G/S, and TKW; NILs targeting the 4BL QTL differed significantly in PH, Y/P, and B/P; NIL pairs targeting the 4AS QTL differed significantly in all the traits; and NIL pairs targeting the 7AL QTL differed significantly in PH. A 90 K SNP genotyping assay of the NILs detected nineteen SNPs associated with fourteen functional genes. Among them, eight candidate genes are related to Rht proteins, four genes are related to hormone pathways and two genes are related to carbohydrate synthesis and transport. By searching the interval marker physical positions, it was found that the four targeted QTL in this study overlapped with eight previously reported QTL for PH, TKW, biomass, and yield. Correlation analysis revealed that PH significantly and positively correlated with B/P and G/S. The SNP and candidate gene information is potentially useful for marker-assisted selection in breeding programs, and the four targeted QTL are proved to be critical genomic regions controlling the investigated agronomic traits, which can be further fine mapped to identify the underlying genes.

株高（PH）与产量相关性状和环境适应密切相关。评估了针对 3AL、4BL、4AS 和 7AL 小麦染色体臂上四个 QTL 的七对近等基因系 (NIL) 的 PH 和四个产量相关性状，包括单株产量 (Y/P)、每穗粒数 ( G/S)、千粒重 (TKW) 和单株生物量 (B/P)。在 NIL 对的测量性状中观察到显着差异。针对 3AL QTL 的 NIL 对在 PH、G/S 和 TKW 方面存在显着差异；靶向 4BL QTL 的 NIL 在 PH、Y/P 和 B/P 方面存在显着差异；靶向 4AS QTL 的 NIL 对在所有性状上均存在显着差异；和 NIL 对针对 7AL QTL 在 PH 方面存在显着差异。NIL 的 90 K SNP 基因分型测定检测到与 14 个功能基因相关的 19 个 SNP。其中，8个候选基因与Rht蛋白相关，4个基因与激素途径相关，2个基因与碳水化合物合成和运输相关。通过搜索区间标记物理位置，发现本研究中的4个目标QTL与之前报道的PH、TKW、生物量和产量的8个QTL重叠。相关分析显示，PH与B/P、G/S呈显着正相关。SNP 和候选基因信息对于育种计划中的标记辅助选择可能有用，并且四个目标 QTL 被证明是控制所研究农艺性状的关键基因组区域，可以进一步精细定位以识别潜在基因。