Danshensu (DSS), the aqueous extracts of Salvia miltiorrhiza has been widely used in biomedical science due to its outstanding capacity in ROS scavenger. However, studies regarding its effects on plant physiology are still scare. In the present study, cucumber seeds were soaked in different concentrations of DSS solution for 24 h before subjected to germination to test its effects on seed germination, and further to explore the metabolic and molecular mechanisms. The results shown that 0.1% DSS had significantly promoted the germination rate, radical length, as well as soluble sugar and H₂O₂ contents. DSS pretreatment upregulated transcript levels of genes involved in SOD, POD, and CAT biosynthesis, concurrent with promoted enzyme activities. In accordance with lower ABA, but higher GA contents, transcript levels of genes involved in ABA and GA biosynthesis were down and upregulated, respectively, in DSS pretreated seeds. DSS pretreatment also stimulated ethylene production and related genes expression levels. These positive effects of DSS on seed germination were confirmed by addition of inhibitors of GA, ABA, NADPH oxidase, and H₂O₂ scavenger. In conclusion, DSS stimulated cucumber seed germination by mediating the regulation of endogenous hormones with promoted ROS levels. Our results highlight the potentiality of DSS in plant science.

丹参（DSS），的水性提取物丹参已广泛由于其在ROS清除剂优秀容量用于生物医学。然而，关于其对植物生理学影响的研究仍很匮乏。在本研究中，黄瓜种子在发芽之前先在不同浓度的DSS溶液中浸泡24小时，以测试其对种子发芽的影响，并进一步探讨其代谢和分子机制。结果表明，0.1％的DSS显着促进了发芽率，自由基长度以及可溶性糖和H ₂ O ₂的发芽。内容。DSS预处理上调涉及SOD，POD和CAT生物合成的基因的转录水平，同时促进酶的活性。根据较低的ABA，但较高的GA含量，DSS预处理种子中与ABA和GA生物合成有关的基因的转录水平分别下调和上调。DSS预处理还刺激了乙烯的产生和相关基因的表达水平。通过添加GA，ABA，NADPH氧化酶和H ₂ O ₂清除剂抑制剂，证实了DSS对种子发芽的这些积极作用。总之，DSS通过介导内源激素的调节与ROS水平的升高来刺激黄瓜种子的发芽。我们的结果突出了DSS在植物科学中的潜力。