This study aimed to optimize pressurized liquid extraction (PLE) of phenolic compounds from the brown seaweed Fucus vesiculosus to obtain extract with high yield, rich in phenolic compounds, with good radical scavenging, and with metal chelating ability. The extraction was optimized with a Box–Behnken design experiment and response surface tool. Optimal condition was found at 137.18 °C, 58.65% v/v ethanol in water and 4.68 min extraction time which resulted in yield of 31.16% dry basis, total phenolic content (TPC) of 3.69 g gallic acid equivalent (100 g dry seaweed)⁻¹, effective concentration for 50% inhibition (EC₅₀) of DPPH radical (92.6 μg mL⁻¹), ABTS radical (2.35 mg mL⁻¹), and metal chelating (1.10 mg mL⁻¹). The TPC value and radical scavenging activity of the PLE extract were insignificantly different from the one obtained from conventional solvent extraction, which proved PLE advantages in terms of shorter time and less solvent requirement. Gallic, protocatechuic, and gentisic acids were identified in the PLE extracts.

这项研究旨在优化从褐藻海藻（Fusus vesiculosus）的酚类化合物的加压液体萃取（PLE），以获得高收率，富含酚类化合物，具有良好的自由基清除和金属螯合能力的提取物。使用Box–Behnken设计实验和响应曲面工具对提取进行了优化。发现最佳条件是在137.18°C，58.65％v / v乙醇在水中和4.68分钟的萃取时间下，其收率为干基的31.16％，总酚含量（TPC）为3.69 g没食子酸当量（100 g干海藻）^-1，对DPPH自由基（92.6μgmL ^-1），ABTS自由基（2.35 mg mL ^-1）的50％抑制（EC ₅₀）的有效浓度）和金属螯合（1.10 mg mL ^-1）。PLE提取物的TPC值和自由基清除活性与常规溶剂提取所得的TPC值和自由基清除活性没有显着差异，这证明PLE在缩短时间和减少溶剂需求方面具有优势。在PLE提取物中鉴定出没食子酸，原儿茶酸和龙胆酸。