Cancer treatment with cisplatin (CP) is associated with adverse side effects on male reproductive tissues. Although beneficial effects of zinc oxide nanoparticles (ZnO NPs) in cancer therapy have received considerable attention, data related to the protective effects of green ZnO NPs against CP-induced male reproductive dysfunctions are limited. Forty-five rats were divided into 9 groups including G1 (control), G2 (sham), G3 (ZnO bulk), G4 (green ZnO NPs), G5 (chemical ZnO NPs), G6 (CP), G7 (CP + ZnO bulk), G8 (CP + green ZnO NPs), and G9 (CP + chemical ZnO NPs). CP was administrated (5 mg/kg/week) for 4 weeks, and animals were simultaneously treated with different forms of ZnO (5 mg/kg/day). Testis histology, sperm parameters, oxidative stress markers, testosterone concentration, and expression of genes related in steroidogenesis were analyzed in different experimental groups. Testis tissue damage and epididymal sperm disorders induced by CP attenuated when animals were treated with different forms of ZnO, especially green ZnO NPs. Decreased testosterone concentration and increased MDA level in CP-treated rats were reversed following administration different forms of ZnO, especially green and chemical ZnO NPs. Co-administration of ZnO NPs to CP-treated rats restored the suppressive effects of CP on activities of antioxidant enzymes (SOD, GPX, CAT) and the transcription of the STAR gene. None of the ZnO forms had a significant regulatory effect on the expression of CYP11A1 in CP-treated rats. The results showed that in most of the evaluated factors, green ZnO NPs showed a greater protective effect than other forms of ZnO.

中文翻译：

---

绿色化学氧化锌纳米粒子对顺铂治疗大鼠睾丸组织学、精子参数、氧化应激标志物和雄激素产生的保护作用

用顺铂 (CP) 治疗癌症与男性生殖组织的不良副作用有关。尽管氧化锌纳米颗粒 (ZnO NPs) 在癌症治疗中的有益作用受到了相当多的关注，但与绿色 ZnO NPs 对 CP 诱导的男性生殖功能障碍的保护作用相关的数据是有限的。45只大鼠分为9组，包括G1（对照）、G2（假）、G3（ZnO块体）、G4（绿色ZnO NPs）、G5（化学ZnO NPs）、G6（CP）、G7（CP + ZnO块）、G8（CP + 绿色 ZnO NP）和 G9（CP + 化学 ZnO NP）。给予 CP (5 mg/kg/周) 4 周，同时用不同形式的 ZnO (5 mg/kg/天) 治疗动物。睾丸组织学、精子参数、氧化应激标志物、睾酮浓度、并分析了不同实验组中类固醇生成相关基因的表达。当动物用不同形式的 ZnO，尤其是绿色 ZnO NPs 处理时，CP 引起的睾丸组织损伤和附睾精子障碍减弱。在施用不同形式的 ZnO，尤其是绿色和化学 ZnO NPs 后，CP 治疗大鼠的睾酮浓度降低和 MDA 水平升高得到逆转。将 ZnO NPs 与 CP 治疗的大鼠共同给药恢复了 CP 对抗氧化酶（SOD、GPX、CAT）活性和 STAR 基因转录的抑制作用。没有一种 ZnO 形式对 CP 处理的大鼠中 CYP11A1 的表达具有显着的调节作用。结果表明，在大多数评估因素中，绿色 ZnO NPs 比其他形式的 ZnO 表现出更大的保护作用。