In response to deal with the increasingly serious environmental problems and shortage of fiber raw materials, biological enzyme pretreatment is an effective way to replace a large number of chemical additives to improve the properties of waste paper fibers. Fiber characterization of old corrugated container bleached pulp with laccase and glycine (Lac/Gly) pretreatment was investigated by means of Fourier transform infrared spectroscopy (FTIR), headspace gas chromatography (HSGC), fiber quality measurements (FQA), X-ray diffraction method (XRD), and atomic force microscope (AFM). Results showed that, compared with the control pulp, the whiteness and brightness of the Lac/Gly-treated pulp increased by 16.17% and 7.41%, respectively. And the FTIR showed that Lac/Gly pretreatment promotes the effective removal of lignin by hydrogen peroxide (H₂O₂) bleaching. The content of carboxyl groups in pulp increased remarkably by 21.92%. The paper physical analysis showed that the paper strength properties have improved remarkably. The fiber quality analyses indicated that the fiber length, coarseness, and curl index changed a little. The XRD analysis showed that the crystallinity decreased by 5.83% due to Lac/Gly treatment. The AFM analysis showed that through Lac/Gly treatment, the lignin and extracts over the fiber surface are decreased significantly.

为了应对日益严重的环境问题和纤维原料的短缺，生物酶预处理是代替大量化学添加剂以改善废纸纤维性能的有效方法。通过傅立叶变换红外光谱（FTIR），顶空气相色谱（HSGC），纤维质量测量（FQA），X射线衍射法研究了使用漆酶和甘氨酸（Lac / Gly）预处理的瓦楞纸箱漂白纸浆的纤维表征（XRD）和原子力显微镜（AFM）。结果表明，与对照纸浆相比，Lac / Gly处理的纸浆的白度和白度分别提高了16.17％和7.41％。FTIR表明Lac / Gly预处理可促进过氧化氢有效去除木质素（H₂ O ₂）漂白。纸浆中的羧基含量显着增加了21.92％。纸的物理分析表明，纸的强度性能得到了显着改善。纤维质量分析表明，纤维长度，粗度和卷曲指数有少许变化。XRD分析表明，由于Lac / Gly处理，结晶度降低了5.83％。AFM分析表明，通过Lac / Gly处理，纤维表面的木质素和提取物明显减少。