Clarias gariepinus growth hormone (CgGH) transgenesis was previously used to develop a population of second-generation (G₂) transgenic mutiara catfish (C. gariepinus). The third generation of these fish (1-month old fingerlings) had 2–3 times improvement in growth compared to non-transgenic fish in the commercial feed test for 6 weeks of rearing. We assessed the impact of CgGH transgene expression on growth and protein and amino acid content of the G₃ generation of these transgenic mutiara catfish relative to non-transgenic catfish. Since variation in composition of feed mixes can affect protein and amino acid content of fish, we tested three mixtures of commercial feed and boiled tuna (Euthynnus affinis): feed A (50:50 feed to tuna), B (65:35), and C (80:20) to transgenic catfish. Feed A* (50:50) was fed as a control to non-transgenic catfish. Feed efficiency, including feed conversion ratio and protein use efficiency (i.e., protein retention and protein productive value), was assessed. Feed efficiency, protein content, and essential amino acid content in G₃ transgenic catfish (feed A and B) were higher than in non-transgenic fish (feed A*). The latter were deficient in lysine and methionine. Transgenic catfish fed with feed C (80:20) showed lysine deficiency and lower growth than fish fed feeds A and B. Feed B (65:35) was the optimal feed mixture utilized; it increased growth, protein levels, and feed conversion efficiency in G₃ catfish. The growth of transgenic fish was higher than non-transgenic fish when supported by feeding with balanced nutrients.

Clarias gariepinus生长激素（Cg GH）转基因以前被用来发展第二代（G ₂）转基因多ti鱼（C. gariepinus）的种群。在商业饲养试验中饲养了6周，与非转基因鱼相比，这些鱼的第三代（1个月大的鱼种）的生长提高了2-3倍。我们评估了Cg GH转基因表达对这些转基因穆迪亚拉on鱼相对于非转基因cat鱼的生长以及G ₃代的蛋白质和氨基酸含量的影响。由于饲料混合物成分的变化会影响鱼的蛋白质和氨基酸含量，因此我们测试了三种商业饲料和水煮金枪鱼的混合物（Euthynnus affinis）：将A（以50:50的比例喂入金枪鱼），B（65:35）和C（80:20）喂入转基因cat鱼。将饲料A *（50:50）作为对照饲喂非转基因cat鱼。评估了饲料效率，包括饲料转化率和蛋白质利用效率（即蛋白质保留率和蛋白质生产价值）。G ₃转基因cat鱼（饲料A和B）的饲料效率，蛋白质含量和必需氨基酸含量高于非转基因鱼（饲料A *）。后者缺乏赖氨酸和蛋氨酸。饲喂饲料C（80:20）的转基因cat鱼比赖氨酸饲料A和B的赖氨酸缺乏，且生长较低。饲料B（65:35）是使用的最佳饲料混合物。它增加了G _3的生长，蛋白质水平和饲料转化效率鲶鱼。当以均衡营养为食时，转基因鱼的生长高于非转基因鱼。