High-temperature stress severely impacts both yield and quality of tomato fruits, and therefore, it is required to develop stress-tolerant cultivars. In the present study, two tomato genotypes, H88-78-1 and CLN-1621, identified through preliminary phenotypic screening were characterized by analysis of molecular, physiological, and biochemical traits in comparison with a susceptible genotype Punjab Chhuhara. Phenotypic stress tolerance of both the genotypes was validated at biochemical level as they showed higher amount of relative water content, photosynthetic pigments, free cellular proline, and antioxidant molecules while less amount of H₂O₂ and electrolyte leakage. Expression analysis of 67 genes including heat shock factors, heat shock proteins, and other stress-responsive genes showed significant up-regulation of many of the genes such as 17.4 kDa class III heat shock protein, HSF A-4a, HSF30, HSF B-2a, HSF24, HSF B-3 like, 18.1 kDa class I HSP like, and HSP17.4 in H88-78-1 and CLN-1621 after exposure to high-temperature stress. These candidate genes can be transferred to cultivated varieties by developing gene-based markers and marker-assisted breeding. This confirms the rapid response of these genotypes to high-temperature stress. All these traits are characteristics of a stress-tolerance and establish them as candidate high-temperature stress-tolerant genotypes that can be effectively utilized in stress tolerance improvement programs.

高温胁迫严重影响番茄果实的产量和品质，因此需要开发抗逆品种。在本研究中，通过初步表型筛选鉴定的两种番茄基因型 H88-78-1 和 CLN-1621 通过与易感基因型 Punjab Chhuhara 相比的分子、生理和生化性状分析来表征。_{两种基因型的表型胁迫耐受性在生化水平上得到验证，因为它们显示出较高的相对水含量、光合色素、游离细胞脯氨酸和抗氧化分子，而 H 2} O ₂的含量较少和电解液泄漏。对包括热休克因子、热休克蛋白和其他应激反应基因在内的 67 个基因的表达分析显示，许多基因如 17.4 kDa III 类热休克蛋白、HSF A-4a、HSF30、HSF B-等显着上调。如图 2a 所示，H88-78-1 和 CLN-1621 暴露于高温应激后，HSF24、HSF B-3 样、18.1 kDa I 类 HSP 样和 HSP17.4。通过开发基于基因的标记和标记辅助育种，可以将这些候选基因转移到栽培品种。这证实了这些基因型对高温胁迫的快速反应。所有这些性状都是胁迫耐受性的特征，并将它们确立为可以在胁迫耐受性改善计划中有效利用的候选高温胁迫耐受基因型。