In recent years, there has been an increasing interest in the remediation of contaminated environments, and a suitable solution is in situ bioremediation. To achieve this, large-scale bacterial biomass production should be sustainable, using economic culture media. The main aim of this study was to optimize the physicochemical conditions for the biomass production of an actinobacterium with well-known bioremediation ability using inexpensive substrates and to scale-up its production in a bioreactor. For this, the growth of four strains of actinobacteria were evaluated in minimal medium with glucose and glycerol as carbon and energy sources. In addition, l-asparagine and ammonium sulfate were assayed as alternative nitrogen sources. The strain Streptomyces sp. A5 showed the highest biomass production in shake-flasks culture using glycerol and ammonium sulfate as carbon and nitrogen sources, respectively. Factorial designs with five factors (glycerol concentration, inoculum size, pH, temperature, and agitation) were employed to optimize the biomass production of Streptomyces sp. A5. The maximum biomass production was obtained using 5 g L⁻¹ of glycerol, 0.25 µL of inoculum, pH 7, 30 °C and 200 rpm. Finally, the production was successfully scaled to a 2 L stirred tank bioreactor.

近年来，人们对污染环境的修复越来越感兴趣，一个合适的解决方案是原位生物修复。为了实现这一目标，大规模的细菌生物质生产应该是可持续的，使用经济的培养基。本研究的主要目的是使用廉价的底物优化具有众所周知的生物修复能力的放线菌的生物质生产的物理化学条件，并在生物反应器中扩大其生产。为此，在以葡萄糖和甘油作为碳和能源的基本培养基中评估了四种放线菌菌株的生长。此外，L-天冬酰胺和硫酸铵被测定为替代氮源。菌株链霉菌sp。A5 分别使用甘油和硫酸铵作为碳源和氮源，在摇瓶培养中显示出最高的生物量产量。采用具有五个因素（甘油浓度、接种量、pH、温度和搅拌）的因子设计来优化链霉菌的生物量生产。A5。使用 5 g L ^-1的甘油、0.25 µL 的接种物、pH 7、30 °C 和 200 rpm 可获得最大的生物质产量。最后，生产成功地扩大到2升搅拌罐生物反应器。