Cytoplasmic lipid droplets are highly dynamic storage organelles; their rapid synthesis, expansion, and degradation, as well as their varied interactions with other organelles allow cells to maintain lipid homeostasis. While the molecular details of lipid droplet dynamics are currently a very active area of investigation, this work has been primarily performed in cultured cells and in vitro systems. By taking advantage of the powerful transgenic and in vivo imaging opportunities afforded by the zebrafish model system, we have built a suite of tools to allow lipid droplets to be studied in real-time from the subcellular to the whole organism level. Fluorescently-tagging the lipid droplet associated proteins, perilipin 2 and perilipin 3, in the endogenous loci, permits visualization of lipid droplets in the intestine, liver, lateral line and adipose tissue. Using these transgenic lines we have found that perilipin 3 is rapidly loaded on intestinal lipid droplets following a high fat meal and then largely replaced by perilipin 2 a few hours later. These powerful new tools will facilitate studies on the role of lipid droplets in different tissues and under different genetic and physiological manipulations.

中文翻译：

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用荧光periplin 2和periplipin 3敲入斑马鱼对体内细胞质脂质滴成像

细胞质脂质滴是高度动态的储存细胞器。它们的快速合成，扩增和降解以及与其他细胞器的多种相互作用，使细胞能够维持脂质体内平衡。虽然脂质小滴动力学的分子细节目前是研究的一个非常活跃的领域，但这项工作主要在培养的细胞和体外系统中进行。通过利用强大的转基因和体内通过斑马鱼模型系统提供的成像机会，我们建立了一套工具，可以实时研究从亚细胞到整个生物体水平的脂滴。荧光标记内源性基因座中与脂滴相关的蛋白质，perilipin 2和perilipin 3，可以观察到肠，肝，侧线和脂肪组织中的脂滴。使用这些转基因品系，我们发现高脂餐后periplipin 3迅速加载到肠道脂质小滴上，然后在数小时后被periplipin 2大量替代。这些功能强大的新工具将有助于研究脂质滴在不同组织中以及在不同遗传和生理操作下的作用。