当前位置: X-MOL 学术Brain Sci. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
AEG-1 Regulates TWIK-1 Expression as an RNA-Binding Protein in Astrocytes
Brain Sciences ( IF 2.7 ) Pub Date : 2021-01-11 , DOI: 10.3390/brainsci11010085
Hyun-Gug Jung , Ajung Kim , Seung-Chan Kim , Jae-Yong Park , Eun Mi Hwang

AEG-1, also called MTDH, has oncogenic potential in numerous cancers and is considered a multifunctional modulator because of its involvement in developmental processes and inflammatory and degenerative brain diseases. However, the role of AEG-1 in astrocytes remains unknown. This study aimed to investigate proteins directly regulated by AEG-1 by analyzing their RNA expression patterns in astrocytes transfected with scramble shRNA and AEG-1 shRNA. AEG-1 knockdown down-regulated TWIK-1 mRNA. Real-time quantitative PCR (qPCR) and immunocytochemistry assays confirmed that AEG-1 modulates TWIK-1 mRNA and protein expression. Electrophysiological experiments further revealed that AEG-1 further regulates TWIK-1-mediated potassium currents in normal astrocytes. An RNA immunoprecipitation assay to determine how AEG-1 regulates the expression of TWIK-1 revealed that AEG-1 binds directly to TWIK-1 mRNA. Furthermore, TWIK-1 mRNA stability was significantly increased upon overexpression of AEG-1 in cultured astrocytes (p < 0.01). Our findings show that AEG-1 serves as an RNA-binding protein to regulate TWIK-1 expression in normal astrocytes.

中文翻译:

AEG-1调节TWIK-1表达作为星形胶质细胞中的一种RNA结合蛋白。

AEG-1(也称为MTDH)在多种癌症中具有致癌作用,由于它参与了发育过程以及炎症性和变性性脑病,因此被认为是多功能调节剂。但是,AEG-1在星形胶质细胞中的作用仍然未知。这项研究旨在通过分析在加扰的shRNA和AEG-1 shRNA转染的星形胶质细胞中的RNA表达模式来研究由AEG-1直接调节的蛋白。AEG-1组合式下调TWIK-1 mRNA。实时定量PCR(qPCR)和免疫细胞化学测定证实AEG-1调节TWIK-1 mRNA和蛋白质表达。电生理实验进一步表明,AEG-1进一步调节正常星形胶质细胞中TWIK-1介导的钾电流。一项用于确定AEG-1如何调节TWIK-1表达的RNA免疫沉淀试验表明,AEG-1直接与TWIK-1 mRNA结合。此外,在培养的星形胶质细胞中过表达AEG-1后,TWIK-1 mRNA的稳定性显着提高(p <0.01)。我们的发现表明,AEG-1是一种RNA结合蛋白,可调节正常星形胶质细胞中TWIK-1的表达。
更新日期:2021-01-11
down
wechat
bug