We characterized two bacteriophages, ΦFG02 and ΦCO01, against clinical isolates of Acinetobacter baumannii and established that the bacterial capsule is the receptor for these phages. Phage-resistant mutants harboured loss-of-function mutations in genes responsible for capsule biosynthesis, resulting in capsule loss and disruption of phage adsorption. The phage-resistant strains were resensitized to human complement, beta-lactam antibiotics and alternative phages and exhibited diminished fitness in vivo. Using a mouse model of A. baumannii infection, we showed that phage therapy was effective.

中文翻译：

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耐噬菌体鲍曼不动杆菌对抗菌剂重新敏感

我们针对鲍曼不动杆菌的临床分离株对两种噬菌体 ΦFG02 和 ΦCO01 进行了表征，并确定细菌荚膜是这些噬菌体的受体。噬菌体抗性突变体在负责胶囊生物合成的基因中存在功能丧失突变，导致胶囊丢失和噬菌体吸附中断。噬菌体抗性菌株对人类补体、β-内酰胺抗生素和替代噬菌体重新敏感，并在体内表现出降低的适应性。使用鲍曼不动杆菌感染的小鼠模型，我们证明噬菌体疗法是​​有效的。