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Bacteriophage-resistant Acinetobacter baumannii are resensitized to antimicrobials
Nature Microbiology ( IF 20.5 ) Pub Date : 2021-01-11 , DOI: 10.1038/s41564-020-00830-7
Fernando Gordillo Altamirano , John H. Forsyth , Ruzeen Patwa , Xenia Kostoulias , Michael Trim , Dinesh Subedi , Stuart K. Archer , Faye C. Morris , Cody Oliveira , Luisa Kielty , Denis Korneev , Moira K. O’Bryan , Trevor J. Lithgow , Anton Y. Peleg , Jeremy J. Barr

We characterized two bacteriophages, ΦFG02 and ΦCO01, against clinical isolates of Acinetobacter baumannii and established that the bacterial capsule is the receptor for these phages. Phage-resistant mutants harboured loss-of-function mutations in genes responsible for capsule biosynthesis, resulting in capsule loss and disruption of phage adsorption. The phage-resistant strains were resensitized to human complement, beta-lactam antibiotics and alternative phages and exhibited diminished fitness in vivo. Using a mouse model of A. baumannii infection, we showed that phage therapy was effective.



中文翻译:

耐噬菌体鲍曼不动杆菌对抗菌剂重新敏感

我们针对鲍曼不动杆菌的临床分离株对两种噬菌体 ΦFG02 和 ΦCO01 进行了表征,并确定细菌荚膜是这些噬菌体的受体。噬菌体抗性突变体在负责胶囊生物合成的基因中存在功能丧失突变,导致胶囊丢失和噬菌体吸附中断。噬菌体抗性菌株对人类补体、β-内酰胺抗生素和替代噬菌体重新敏感,并在体内表现出降低的适应性。使用鲍曼不动杆菌感染的小鼠模型,我们证明噬菌体疗法是​​有效的。

更新日期:2021-01-11
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