Abstract

The diseases and disorders of the cornea may lead to blindness, and cornea transplantation has been an effective treatment in this regard. However, lack of cornea throughout the world makes this treatment difficult. Therefore, the first goal of the present study is to make a chitosan/gelatin hyaline film with NHS and EDC crosslinkers for transplanting the epithelial cells of the cornea. Two solutions of gelatin and chitosan were mixed homogeneously before crosslinking in ratios of 20/80, 30/70, 40/60, and 50/50 (Gel/Chi). After 24 hours, they were put in an oven to dry, then EDC and NHS were added to the mixture as crosslinker. Corneal epithelial cell morphology was assessed qualitatively and compared between different ratios of chitosan and gelatin using corneal epithelial cell culture tests. Mechanical and optical analysis were performed; the results implied an increase in chitosan concentration could improve transparency and biocompatibility of produced samples. Fourier Transform Infrared Spectroscopy (FTIR) and SEM analysis were done for the chemical and morphological characterization of fabricated films. Moreover, the presented results revealed that increment in chitosan increased water permeability and degradability of samples. Besides, MTT essays showed that with increment in chitosan concentration, cell proliferation and cell viability were significantly improved.

摘要

角膜的疾病和失调可能导致失明，并且角膜移植在这方面一直是有效的治疗方法。但是，全世界缺乏角膜使这种治疗变得困难。因此，本研究的第一个目标是制备具有NHS和EDC交联剂的壳聚糖/明胶透明膜，用于移植角膜上皮细胞。明胶和壳聚糖的两种溶液在交联之前以20 / 80、30 / 70、40 / 60和50/50（Gel / Chi）的比例均匀混合。24小时后，将它们放在烤箱中干燥，然后将EDC和NHS作为交联剂添加到混合物中。定性评估角膜上皮细胞形态，并使用角膜上皮细胞培养测试比较不同比例的壳聚糖和明胶之间的关系。进行了机械和光学分析。结果表明，壳聚糖浓度的增加可以提高所生产样品的透明度和生物相容性。进行了傅里叶变换红外光谱（FTIR）和SEM分析，以对制成膜的化学和形态进行表征。此外，提出的结果表明，壳聚糖的增加增加了样品的透水性和降解性。此外，MTT论文表明，随着壳聚糖浓度的增加，细胞增殖和细胞活力得到显着改善。提出的结果表明，壳聚糖的增加增加了样品的透水性和降解性。此外，MTT论文表明，随着壳聚糖浓度的增加，细胞增殖和细胞活力得到显着改善。提出的结果表明，壳聚糖的增加增加了样品的透水性和降解性。此外，MTT论文表明，随着壳聚糖浓度的增加，细胞增殖和细胞活力得到显着改善。