ABSTRACT

MicroRNA plays an important regulatory role in the development of all organisms, including hair follicle development. In order to improve domestic cashmere yield, the role of miRNA in hair follicle cycle has become a research hotspot. However, the molecular and cellular mechanisms by which miRNA-203 regulates hair follicle development are not completely understood. In this study, we found that the relevant target genes of miRNA-203 (DDOST and NAE1) were less expressed in telogen by qPCR and Immunoblotting analysis, contrary to the expression mode of miRNA-203. The Dual-Luciferase reporter assay was used to verify the correlation between miRNA-203 and its target gene expression. The results showed that miRNA-203 specifically binds to the 3 ‘UTR of DDOST and NAE1, and the expression of miRNA-203 significantly down-regulates the expression of DDOST and NAE1 mRNA and protein. Therefore, this study demonstrates that miRNA-203 may regulate hair follicle development in Cashmere goats by targeting DDOST and NAE1.

摘要

MicroRNA在包括毛囊发育在内的所有生物的发育中起着重要的调节作用。为了提高国内羊绒产量，miRNA在毛囊周期中的作用已成为研究热点。但是，miRNA-203调节毛囊发育的分子和细胞机制尚不完全清楚。在这项研究中，我们发现通过qPCR和免疫印迹分析，miRNA-203的相关靶基因（DDOST和NAE1）在端粒中的表达较少，这与miRNA-203的表达方式相反。使用双重萤光素酶报告基因测定法验证了miRNA-203及其靶基因表达之间的相关性。结果表明，miRNA-203与DDOST和NAE1的3'UTR特异性结合，miRNA-203的表达显着下调DDOST和NAE1 mRNA和蛋白的表达。因此，这项研究表明，miRNA-203可能通过靶向DDOST和NAE1来调节羊绒山羊的毛囊发育。