Abstract

Cinnamaldehyde (CA) is one of the major active pharmaceutical ingredient of cinnamon bark. Hydrodistillation (HD) is usually used in CA extraction, however, the extraction yield is lower. The cell wall is a key factor limiting the extraction of essential oils. In-situ reactive heat breaking cell wall (RHB) could destroy the cell wall, which was conducive to the diffusion of CA. The aim of this work was to examine the effect of RHB pretreatment to HD extraction. Response surface methodology (RSM) was used to optimize RHB pretreatment parameters, and Box-Behnken Design (BBD) method was performed to evaluate the effects of different operating parameters. The maximum yield was increased to 3.31 ± 0.11% (w/w) from 2.08 ± 0.042% (w/w) after RSM optimization. Scanning electron microscopic (SEM) analysis showed that RHB destroyed and disrupted the cell wall of cinnamon bark. The GC analysis demonstrated that the purity of cinnamaldehyde was improved and no new components were presented in the extraction product from the cinnamon via RHB pretreatment. In conclusion, RHB is an effective pretreatment method for the CA extraction, and also may be used in the other herbal medicine extraction.

摘要

肉桂醛（CA）是肉桂皮的主要活性药物成分之一。CA萃取通常使用加氢蒸馏（HD），但是萃取收率较低。细胞壁是限制精油提取的关键因素。原位反应热破碎细胞壁（RHB）可能破坏细胞壁，这有助于CA的扩散。这项工作的目的是检查RHB预处理对HD提取的影响。响应面方法（RSM）用于优化RHB预处理参数，Box-Behnken设计（BBD）方法用于评估不同操作参数的影响。最大产量从2.08±0.042％（w / w）增加到3.31±0.11％（w / w）），然后进行RSM优化。扫描电镜（SEM）分析表明，RHB破坏并破坏了肉桂皮的细胞壁。GC分析表明，肉桂醛的纯度得到了改善，并且通过RHB预处理从肉桂中提取的产品中没有新成分存在。总之，RHB是CA提取的有效预处理方法，也可用于其他草药提取。