ABSTRACT

A Calothrix-like cyanobacterial strain was isolated from a sample collected from the entrance zone of Shumyaschiy Ponor cave, Chelyabinsk Region, South Urals, Russia. This isolate showed diagnostic features of the recently described genus Roholtiella, i.e. chains of arthrospores and thalli not tapering to a hair. Molecular analyses based on 16S rRNA gene, 16S–23S internal transcribed spacer (ITS) sequences, and spacer secondary structure revealed that our isolate was a distinct lineage within the genus clade that we describe as Roholtiella mixta sp. nov. High-performance liquid chromatography identified β-carotene, zeaxanthin, 3-hydroxyechinenone (4-keto-3-hydroxy-β-carotene), echinenone, caloxanthin, β-cryptoxanthinoleate, and antheraxanthin in R. mixta. Antheraxanthin was recorded for the second time only in cyanobacteria species.

摘要

从俄罗斯南乌拉尔州车里雅宾斯克州Shumyaschiy Ponor洞穴入口区采集的样品中分离出一种类似于Calothrix的蓝细菌菌株。该分离物显示了最近描述的罗氏杆菌属的诊断特征，即节孢和链状藻的链没有逐渐变细。基于16S rRNA基因，16S–23S内部转录间隔区（ITS）序列和间隔区二级结构的分子分析表明，我们的分离株是我们所描述的Roholtiella mixta sp。属进化枝中的独特谱系。十一月 高效液相色谱法鉴定了其中的β-胡萝卜素，玉米黄质，3-羟基七烯酮（4-酮-3-羟基-β-胡萝卜素），海胆烯酮，钙黄素，β-隐黄嘌呤油酸酯和花药黄嘌呤。R. mixta。仅在蓝细菌物种中第二次记录了花药黄素。