Abstract

Aim: 2,6-Di-isopropylphenol (propofol) is an intravenous general anesthetic widely used in the operating room for general anesthesia and in the intensive care unit for sedation. The mouse air pouch model is versatile in studying the anti-inflammatory effect of a drug on a local inflammation, which is induced by a variety of substances. In this study, using the carrageenan-induced air pouch inflammation model, we tested whether propofol mitigates inflammation occurring locally in the mouse air pouch.

Methods: Carrageenan-induced air pouch inflammation model.

Results: Propofol inhibited the production of tumor necrosis factor (TNF)-α and interleukin (IL)-6 in the pouch. Propofol also inhibited the production of neutrophil chemokines, KC and MIP-2, and decreased the number of both Ly-6G⁺/CD11b⁺ cells (assumed to be primarily neutrophils) and Ly-6G⁻/CD11b⁺ cells (assumed to be monocytes/macrophages), recruited into the pouch at 3 h after injection of carrageenan.

Conclusion: Propofol has an anti-inflammatory property in the carrageenan-induced mouse air pouch local inflammation model, by inhibiting the production of pro-inflammatory cytokines (TNF-α and IL-6), as well as by inhibiting the production of chemokines (KC and MIP-2), which might be associated with the inhibition of intra-pouch recruitment of white blood cells.

摘要

目的： 2,6-二异丙基苯酚（丙泊酚）是一种静脉全身麻醉剂，广泛用于手术室进行全身麻醉和重症监护室进行镇静。小鼠气囊模型在研究药物对由多种物质诱发的局部炎症的抗炎作用方面具有多种用途。在这项研究中，使用角叉菜胶诱导的气袋炎症模型，我们测试了异丙酚是否可以减轻小鼠气袋中局部发生的炎症。

方法：角叉菜胶诱发的气袋炎症模型。

结果：异丙酚抑制了小袋中肿瘤坏死因子（TNF）-α和白介素（IL）-6的产生。丙泊酚也抑制了生产嗜中性粒细胞趋化因子，KC和MIP-2的，并且降低两者Ly的-6G的数目⁺ / CD11b的⁺细胞（假定为主要中性粒细胞）和L y-6G ^- / CD11b的⁺细胞（假定是单核细胞/巨噬细胞），在注入角叉菜胶后3小时进入小袋。

结论：丙泊酚通过抑制促炎细胞因子（TNF-α和IL-6）的产生以及抑制趋化因子的产生，在角叉菜胶诱导的小鼠气袋局部炎症模型中具有抗炎作用。 KC和MIP-2），可能与抑制白细胞的袋内募集有关。