ABSTRACT

This study aimed to evaluate the role of biochar properties and application rate in the alleviation of negative impacts of salinity stress on the growth of salt-sensitive common bean (Phaseolus vulgaris L.). The biochar was obtained by slow pyrolysis of olive pruning at 400°C and characterized as high alkaline (9.3) and having a high cation exchange capacity (CEC) and low electrical conductivity (EC) with high porous surface morphology. Plants were grown in the greenhouse and subjected to different amounts of salt (0, 25, 50 mM NaCl) and biochar rate (0, 5, 15 t/ha). Salinity stress reduced shoot growth, biomass accumulation, total chlorophyll and carotenoid content, these parameters were ameliorated by biochar. Adverse effects of salinity on pod settings were also mitigated by biochar supplements. Salinity triggered oxidative stress monitored by increasing hydrogen peroxide (H₂O₂) content and cell membrane permeability of leaves were mitigated by only 5 t/ha biochar application in contrast 15 t/ha biochar extended oxidative stress level. Our results showed that biochar application may be a promising strategy to increase common bean growth under saline soils due to its specific physicochemical properties, particularly high Na⁺ adsorption capacity. However, the higher biochar application rate may augment the adverse effects of soil salinity for salt-sensitive bean growth due to its contribution to the cumulative EC level of the soil.

摘要

本研究旨在评估生物炭特性和施用量在缓解盐分胁迫对盐敏感型普通菜豆（菜豆）生长的负面影响中的作用。L.）。该生物炭是通过在400°C下对橄榄修剪进行缓慢热解而获得的，其特征在于高碱性（9.3），高阳离子交换容量（CEC）和低电导率（EC）和高多孔表面形态。植物在温室中生长，并经受不同量的盐（0、25、50 mM NaCl）和生物炭比率（0、5、15 t / ha）。盐分胁迫降低了枝条的生长，生物量的积累，总叶绿素和类胡萝卜素的含量，这些参数可以通过生物炭改善。生物炭补充剂也减轻了盐度对豆荚环境的不利影响。通过增加过氧化氢（H ₂ O _2）监测盐度触发的氧化应激）的含量和叶片的细胞膜通透性仅通过施用5 t / ha生物炭来缓解，而15 t / ha生物炭扩展了氧化应激水平。我们的研究结果表明，由于生物炭的特殊理化特性，特别是高的Na ⁺吸附能力，生物炭的应用可能是增加盐碱土下普通豆类生长的一种有前途的策略。但是，较高的生物炭施用量可能会增加盐分对盐敏感型豆生长的不利影响，因为盐分对土壤累积的EC水平有贡献。