Ring assembly of c subunits of F 0 F 1 ‐ATP synthase in Propionigenium modestum requires YidC and UncI following MPIase‐dependent membrane insertion,FEBS Letters

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Ring assembly of c subunits of F 0 F 1 ‐ATP synthase in Propionigenium modestum requires YidC and UncI following MPIase‐dependent membrane insertion
FEBS Letters ( IF 3.0 ) Pub Date : 2021-01-29 , DOI: 10.1002/1873-3468.14036
Hanako Nishikawa ₁ , Kotoka Kanno ₂ , Yuta Endo ₁ , Ken-Ichi Nishiyama _{1,

2}

Affiliation

The c subunits of F0 F1 -ATP synthase (F0 c) assemble into a ring structure, following membrane insertion that is dependent on both glycolipid MPIase and protein YidC. We analyzed the insertion and assembly processes of Propionigenium modestum F0 c (Pm-F0 c), of which the ring structure is resistant to SDS. Ring assembly of Pm-F0 c requires P. modestum UncI (Pm-UncI). Ring assembly of in vitro synthesized Pm-F0 c was observed when both YidC and Pm-UncI were reconstituted into liposomes of E. coli phospholipids. Under the physiological conditions where spontaneous insertion had been blocked by diacylglycerol, MPIase was necessary for Pm-F0 c insertion allowing the subsequent YidC/Pm-UncI-dependent ring assembly. Thus, we have succeeded in the complete reconstitution of membrane insertion and subsequent ring assembly of Pm-F0 c.

中文翻译：

在 MPIase 依赖性膜插入后，Propionigenium 中 F 0 F 1 -ATP 合酶 c 亚基的环组装需要 YidC 和 UncI

在依赖糖脂 MPIase 和蛋白质 YidC 的膜插入后，F0 F1 -ATP 合酶 (F0 c) 的 c 亚基组装成环状结构。我们分析了 Propionigenium mediumum F0 c (Pm-F0 c) 的插入和组装过程，其中环结构对 SDS 具有抗性。Pm-F0 c 的环组装需要 P.温和 UncI (Pm-UncI)。当 YidC 和 Pm-UncI 重组为大肠杆菌磷脂的脂质体时，观察到体外合成的 Pm-F0 c 的环组装。在自发插入被甘油二酯阻断的生理条件下，MPIase 是 Pm-F0 c 插入所必需的，允许随后的 YidC/Pm-UncI 依赖性环组装。因此，我们成功地完全重建了膜插入和随后的 Pm-F0 c 环组装。

更新日期：2021-01-29

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