Establishing the long-term effects of different types of animal manures on soil organic carbon (SOC) stocks has both agronomic and environmental implications. However, only a few studies have analyzed the effects of the types of animal manure on SOC storage in subsurface soil layers. Furthermore, the effect of animal manure on labile and stable soil organic matter (SOM) fractions has been scarcely reported for subtropical no-till soils. We evaluated the effect of animal manure application in the short and long term (4 and 12 years) on SOC storage at different depths throughout a 0–70 cm soil profile and on the physical fractions of SOM in the 0–20 cm layer of a sandy Alfisol cropped under no-tillage. The study involved five treatments: pig deep-litter (PDL), cattle slurry (CS), pig slurry (PS), mineral fertilizer (MF) and a control without fertilization (CL). The SOC stock in the soil profile (0–70 cm) was influenced by manure application in only the long term, which was due to high SOC accumulation in the surface (0–20 cm) in the short-and long-term. The PDL and CS applications resulted in 16% (8.6 Mg ha⁻¹) and 10% (5.2 Mg ha⁻¹) increases in the SOC stock, respectively, compared with those in the CL, but only PDL resulted in greater SOC stocks than MF. These results are related to the greater C addition and higher SOC retention coefficient with the PDL application (17%) than with the CS (15%) and PS (7%) applications. Most of the manure-induced changes were observed in the silt plus clay-sized heavy fraction (SCHF), with PDL and CS being more efficient at increasing SOC storage as organic-mineral complexes than PS or MF in the 0–20 cm soil layer. However, in the subsurface layer (20–70 cm), manure caused a decrease in SOC compared with that in the CL, resulting in negative SOC storage rates in this layer, which partially or completely neutralized the SOC storage rates observed in the surface layer (0–20 cm). In the long term, this condition resulted in SOC storage rates in the 0–70 cm soil profile of 0.48 Mg ha⁻¹ year⁻¹ for PDL, 0.21 Mg ha⁻¹ year⁻¹ for CS, −0.03 Mg ha⁻¹ year⁻¹ for PS and 0.01 Mg ha⁻¹ year⁻¹ for MF. These results indicate that PDL and CS are suitable alternatives to MF for increasing the SOC stock and that the SOC retention is greater with solid manure than with liquid manure (PDL vs CS and PS) and with CS than with PS. Moreover, the results reveal the importance of monitoring SOC changes in the long term and within deep soil layers to better quantify the effects of successive applications of animal manure on SOC storage potential in subtropical sandy soils under no-tillage.

建立不同类型的动物粪便对土壤有机碳（SOC）存量的长期影响对农业和环境都有影响。但是，只有很少的研究分析了动物粪便类型对地下土壤层中SOC储存的影响。此外，对于亚热带免耕土壤，几乎没有报道过动物肥料对不稳定和稳定的土壤有机质（SOM）组分的影响。我们评估了短期和长期（4年和12年）施用动物粪肥对整个0–70 cm土壤剖面中不同深度的SOC储存以及0–20 cm土层中SOM物理分数的影响。免耕种植的沙质Alfisol。该研究涉及五种处理方法：猪深胎（PDL），牛粪（CS），猪粪（PS），矿物肥料（MF）和不施肥的对照（CL）。土壤剖面中的SOC储量（0–70 cm）仅在长期内受到施肥的影响，这是由于短期和长期在表面（0–20 cm）中高的SOC积累所致。PDL和CS应用导致16％（8.6 Mg ha^-1）和10％（5.2 Mg ha ^-1），与CL相比，SOC的库存量分别增加，但是只有PDL导致SOC的库存量比MF更大。这些结果与PDL应用（17％）比CS（15％）和PS（7％）应用更大的C添加量和更高的SOC保留系数有关。大多数肥料引起的变化都在粉砂加粘土大小的重质分数（SCHF）中观察到，在0-20厘米的土壤层中，PDL和CS作为有机矿物复合物在增加SOC储存方面比PS或MF更有效。 。但是，在地下层（20-70厘米）中，粪肥导致土壤有机碳含量比土壤有机碳降低，导致该层的有机碳储量为负值，这部分或完全抵消了在表面层中观测到的有机碳储量。 （0–20厘米）。在长期，^-1年^-1为客运专线，0.21镁公顷^-1年^-1为CS，-0.03镁公顷^-1年^-1的PS和0.01镁公顷^-1年^-1的MF。这些结果表明，PDL和CS是用于增加SOC储量的MF的合适替代品，并且固态肥料的SOC保留大于液态肥料（PDL vs CS和PS），CS的PS保留大于PS。此外，结果显示了长期监测土壤深层SOC的重要性，以更好地量化动物粪肥连续施用对免耕亚热带沙质土壤SOC储存潜力的影响。