Bombyx mori was used as a model to evaluate the reproductive toxicity of Novaluron in insects. Morphological analyses of the testes and ovaries of B. mori throughout their life cycle revealed important alterations in the germ and somatic cells involved in spermatogenesis and oogenesis. We observed in all testicular developmental phases that Novaluron affected not only the organization, distribution and development of the cysts containing male germ cells, but also the morphological features of cell death. Similar cellular characteristics were found in the treated B. mori ovaries, suggesting the occurrence of cell death in both organs, in addition to a significant reduction in oviposition of eggs by female moths. We demonstrated reproductive toxicity of Novaluron to the nontarget beneficial insect silkworm, thus providing a theoretical basis for revealing the reproductive toxicity of this insecticide to other nontarget beneficial insects.

家蚕被用作评估Novaluron在昆虫中的生殖毒性的模型。在整个生命周期中对家蚕的睾丸和卵巢进行形态学分析表明，参与精子发生和卵子发生的生殖细胞和体细胞发生了重要变化。我们在所有睾丸发育阶段都观察到，Novaluron不仅影响包含雄性生殖细胞的囊肿的组织，分布和发育，而且还影响细胞死亡的形态特征。在处理的家蚕中发现相似的细胞特征卵巢，提示在雌性蛾中卵子的产卵量显着降低之外，在两个器官中都发生了细胞死亡。我们证明了Novaluron对非目标有益昆虫蚕的生殖毒性，从而为揭示该杀虫剂对其他非目标有益昆虫的生殖毒性提供了理论基础。