In this study, a distinct inoculum was investigated as an isolated variable within sequencing batch reactors via a comparison of the 4-fluoroaniline (4-FA) or 2,4-difluoroaniline (2,4-DFA) removal amounts. The inocula were derived from a treatment plant for treating pharmaceutical wastewater plus a small amount of municipal sewage (PMS), a treatment plant for treating fluoridated hydrocarbon wastewater (FHS), and a treatment plant for treating the comprehensive wastewater in an industrial park (CIS). There were slight differences among the degradation patterns of the 4-FA for the three inocula, whether during the enrichment period or the high concentration shock period. In contrast, it was observed that the degradation efficiency of 2,4-DFA initially varied with the inocula. The FHS-derived inoculum was determined to be optimal, exhibiting the earliest degradation reaction only after an acclimation of 7 days had the highest degradation rate constant of 0.519 h⁻¹, and had the fastest recovery time of three weeks after high concentration shock. Additionally, compared with the PMS-derived inoculum, the CIS-derived inoculum exhibited an earlier degradation reaction within three weeks, and a higher microbial diversity, but a lower shock resistance and degradation rate constant of 0.257 h⁻¹. High-throughput sequencing demonstrated that each final consortium was different in composition, and the microbial consortia developed well on the inoculum and substrate. In comparison of the similarity among the three 2,4-DFA enrichment cultures, the higher similarity (63.9–70.0%) among three final consortia enriching with 4-FA was observed. The results indicated that the inoculum played an important role in the degradation of FAs and the microbial bacterial communities of final consortia, and the effect extent might well depend on the fluorinated level of FAs.

在这项研究中，通过比较4-氟苯胺（4-FA）或2,4-二氟苯胺（2,4-DFA）的去除量，研究了分批批量反应器中不同接种物的分离变量。接种物来自用于处理制药废水加少量城市污水（PMS）的处理厂，用于处理氟化碳氢化合物废水（FHS）的处理厂以及用于处理工业园区中综合废水的处理厂（CIS） ）。无论是在富集期还是在高浓度震荡期，三种接种物的4-FA降解模式之间都存在细微差异。相反，观察到2，4-DFA的降解效率最初随接种物而变化。FHS衍生的接种物被确定为最佳，^-1，在高浓度电击后恢复时间最快，为三周。另外，与PMS来源的接种物相比，CIS来源的接种物在三周内表现出更早的降解反应，并且具有更高的微生物多样性，但是更低的抗冲击性和降解速率常数为0.257h ^-1。高通量测序表明，每个最终的财团在组成上都不同，并且微生物财团在接种物和底物上发育良好。比较三种2，4-DFA富集培养之间的相似性，观察到三个富集4-FA的最终财团之间的相似性更高（63.9–70.0％）。结果表明，接种物在FAs的降解和最终菌群的微生物群落中起着重要作用，其影响程度可能取决于FAs的氟化水平。