This paper studies the impacts of the construction of a new highway on the built-up land use dynamics in the southern neighboring area of Beirut in Lebanon, whereas the impact of transport infrastructure on land use dynamics has been widely studied, few examples are found for developing countries where data are not widely available. Moreover, it is difficult to assess the impact of the infrastructure among several external and internal factors. Areas of built-up land use in Beirut Southern entrance in Lebanon were extracted from land use classification maps for years 1985 to 2019 using 30 m resolution satellite reflectance images in ArcGIS software. To compute an accessibility index to the population, average car travel speeds were determined for major local roads and population distributions were collected for years 2004 to 2019. Results revealed that after the completion of highway’s construction, the development of built-up land use has increased by 13.9%, but also that the land use dynamics depends highly on the unexpected arrival of Syrian refugees between years 2011 and 2013. The results were analyzed with the help of several interviews of stakeholders.