Significance: Particle field holography is a versatile technique to determine the size and distribution of moving or stationary particles in air or in a liquid without significant disturbance of the sample volume. Although this technique is applied in biological sample analysis, it is limited to small sample volumes, thus increasing the number of measurements per sample. In this work, we characterize the maximum achievable volume limit based on the specification of a given sensor to realize the development of a potentially low-cost, single-shot, large-volume holographic microscope. Aim: We present mathematical formulas that will aid in the design and development and improve the focusing speed for the numerical reconstruction of registered holograms in particle field holographic microscopes. Our proposed methodology has potential application in the detection of Schistosoma haematobium eggs in human urine samples. Approach: Using the Fraunhofer holography theory for opaque objects, we derived an exact formula for the maximum diffraction-limited volume for an in-line holographic setup. The proof-of-concept device built based on the derived formulas was experimentally validated with urine spiked with cultured Schistosoma haematobium eggs. Results: Results obtained show that for urine spiked with Schistosoma haematobium eggs, the volume thickness is limited to several millimeters due to scattering properties of the sample. The distances of the target particles could be estimated directly from the hologram fringes. Conclusion: The methodology proposed will aid in the development of large-volume holographic microscopes.

中文翻译：

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用于生物样品分析的大体积全息成像

意义：粒子场全息术是一种通用技术，可以确定空气或液体中移动或静止粒子的大小和分布，而不会显着扰乱样品体积。尽管该技术应用于生物样品分析，但仅限于小样品量，因此增加了每个样品的测量次数。在这项工作中，我们根据给定传感器的规格来表征最大可实现的体积限制，以实现潜在低成本、单次拍摄、大体积全息显微镜的开发。目的：我们提出的数学公式将有助于设计和开发并提高粒子场全息显微镜中配准全息图数值重建的聚焦速度。我们提出的方法在检测人类尿液样本中的血吸虫虫卵方面具有潜在的应用价值。方法：使用不透明物体的弗劳恩霍夫全息理论，我们推导出了一个精确的公式，用于在线全息设置的最大衍射极限体积。基于推导出的公式构建的概念验证设备通过添加有培养的血吸虫血吸虫卵的尿液进行了实验验证。结果：获得的结果表明，对于掺有血吸虫虫卵的尿液，由于样品的散射特性，其体积厚度被限制在几毫米以内。目标粒子的距离可以直接从全息图条纹中估计出来。结论：所提出的方法将有助于开发大体积全息显微镜。