Glioblastoma multiforme (GBM) is the most common and biologically aggressive brain tumour. The current standard therapy for GBM consists in surgical resection, followed by radiotherapy and chemotherapy. Yet, the treatment is limited due to the area for the surgical resection and for the inability of some drugs to cross the brain blood barrier, leading to a general prognostic of no more than a year. Cold atmospheric plasma (CAP) is a new approach in the treatment of this challenging disease. CAP interaction with cells is dependent on physical and chemical factors, with different plasma discharges, cell type, and culture conditions leading to different CAP activity. Considering the plasma self-adaptation that different plasma discharge modes can undergo, which leads to different interaction plasma/cells, the characterization of a new device is essential. In this study we analysed the effect of a novel large pin-to-plate non-thermal atmospheric plasma on U-251 MG cells under different conditions. The analysis of reactive oxygen and nitrogen species (RONS) on plasma, media and cells were also assessed. We were able to demonstrate that the pin-to-plate device is cytotoxic to GBM cells in a dose, time and ROS dependent manner. The measurements of RONS on plasma/media also give us an insight on the chemical effect of this novelty device, and the possibility to better understand the use of this device as a promising GBM therapy.

中文翻译：

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销电极反应器：一种新型的冷常压等离子体装置及其在胶质母细胞瘤治疗中的潜力

多形胶质母细胞瘤（GBM）是最常见的生物攻击性脑肿瘤。GBM当前的标准疗法包括手术切除，放射疗法和化学疗法。然而，由于手术切除的区域以及某些药物无法穿过脑血屏障，治疗的局限性，导致总体预后不超过一年。大气冷血浆（CAP）是治疗这种具有挑战性的疾病的一种新方法。CAP与细胞的相互作用取决于物理和化学因素，不同的血浆放电，细胞类型和培养条件会导致CAP活性不同。考虑到不同的等离子体放电模式可能经历的等离子体自适应，这导致等离子体/细胞之间的相互作用不同，新设备的表征至关重要。在这项研究中，我们分析了新型大针对板非热大气等离子体在不同条件下对U-251 MG细胞的影响。还评估了血浆，培养基和细胞上活性氧和氮物种（RONS）的分析。我们能够证明针板式设备对GBM细胞具有剂量，时间和ROS依赖性的细胞毒性。在血浆/介质上对RONS的测量还使我们对这种新颖设备的化学作用有了更深入的了解，并有可能更好地了解将此设备用作有前途的GBM治疗方法。还评估了培养基和细胞。我们能够证明针板式设备对GBM细胞具有剂量，时间和ROS依赖性的细胞毒性。在血浆/介质上对RONS的测量还使我们对这种新颖设备的化学作用有了更深入的了解，并有可能更好地了解将此设备用作有前途的GBM治疗方法。还评估了培养基和细胞。我们能够证明针板式装置对GBM细胞具有剂量，时间和ROS依赖性的细胞毒性。在血浆/介质上对RONS的测量还使我们对这种新颖设备的化学作用有了更深入的了解，并有可能更好地了解将此设备用作有前途的GBM治疗方法。