Genome sequence assemblies provide the basis for our understanding of biology. Generating error-free assemblies is therefore the ultimate, but sadly still unachieved goal of a multitude of research projects. Despite the ever-advancing improvements in data generation, assembly algorithms and pipelines, no automated approach has so far reliably generated near error-free genome assemblies for eukaryotes. Whilst working towards improved datasets and fully automated pipelines, assembly evaluation and curation is actively used to bridge this shortcoming and significantly reduce the number of assembly errors. In addition to this increase in product value, the insights gained from assembly curation are fed back into the automated assembly strategy and contribute to notable improvements in genome assembly quality. We describe our tried and tested approach for assembly curation using gEVAL, the genome evaluation browser. We outline the procedures applied to genome curation using gEVAL and also our recommendations for assembly curation in a gEVAL-independent context to facilitate the uptake of genome curation in the wider community.

中文翻译：

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通过管理显着提高基因组组装的质量


基因组序列组装为我们理解生物学提供了基础。因此，生成无错误的组件是最终的目标，但遗憾的是，许多研究项目仍未实现这一目标。尽管数据生成、组装算法和管道不断进步，但迄今为止还没有自动化方法能够可靠地为真核生物生成近乎无差错的基因组组装。在努力改进数据集和全自动管道的同时，积极使用装配评估和管理来弥补这一缺点，并显着减少装配错误的数量。除了产品价值的增加之外，从组装管理中获得的见解也会反馈到自动化组装策略中，并有助于显着提高基因组组装质量。我们描述了使用基因组评估浏览器 gEVAL 进行尝试和测试的组装管理方法。我们概述了使用 gEVAL 应用于基因组管理的程序，以及我们在独立于 gEVAL 的背景下进行组装管理的建议，以促进更广泛的社区采用基因组管理。