The noncoding RNAs play important role in growth and development of mammalian skeletal muscle. Recent work has shown that circRNAs are abundant in skeletal muscle tissue, with significant changes in their expression patterns during muscle development and aging. We identified a novel circRNA called circARID1A that is highly expressed in mice skeletal muscle compare to its linear transcript. Experiments shown that circARID1A significantly inhibited the process of C2C12 cell proliferation and promoted its differentiation. Interactions between circRNA and miRNA were screened by miRNA gene chip sequencing. The results indicated that circARID1A can sponge miR-6368, which was further verified by miRNA sensor and other experiments. Besides, miR-6368 is a commonly expressed miRNA that regulates the expression of several target genes including Tlr4. A mouse model of skeletal muscle injury was successfully established to explore the role of circARID1A in skeletal muscle development and regeneration in vivo. Moreover, we found the overexpression of circARID1A significantly promoted the regeneration of skeletal muscle. The results of our study suggest that circARID1A may regulate skeletal muscle cell development and regeneration by sponging miR-6368.

中文翻译：

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CircARID1A 通过充当 miR-6368 的海绵来调节小鼠骨骼肌再生

非编码 RNA 在哺乳动物骨骼肌的生长和发育中起重要作用。最近的工作表明，circRNAs 在骨骼肌组织中含量丰富，在肌肉发育和衰老过程中它们的表达模式发生了显着变化。我们鉴定了一种称为 circARID1A 的新型 circRNA，与其线性转录物相比，它在小鼠骨骼肌中高度表达。实验表明circARID1A显着抑制C2C12细胞增殖过程并促进其分化。通过miRNA基因芯片测序筛选circRNA和miRNA之间的相互作用。结果表明circARID1A可以海绵化miR-6368，miRNA传感器等实验进一步验证了这一点。此外，miR-6368 是一种普遍表达的 miRNA，可调节包括 Tlr4 在内的多种靶基因的表达。成功建立骨骼肌损伤小鼠模型，探索circARID1A在体内骨骼肌发育和再生中的作用。此外，我们发现circARID1A的过表达显着促进了骨骼肌的再生。我们的研究结果表明，circARID1A 可能通过海绵 miR-6368 调节骨骼肌细胞的发育和再生。