As one of the piscine rhabdoviruses, Siniperca chuatsi rhabdovirus (SCRV) has caused considerable losses to mandarin fish aquaculture industry. RNA‐seq, as efficient transcriptome research method, has been widely used to study the immune response of fish to pathogens. This study reported the effect of SCRV infection at 0, 24 and 60 hr on S. chuatsi at the transcriptome level. A total of 61,527 unigenes with high quality were obtained, and 3,095, 1,854 and 227 differentially expressed genes (DEGs) were labelled between the Sc24 and Sc0 groups, the Sc60 and Sc0 groups and the Sc60 and Sc24 groups, respectively. Genes involved in innate and adaptive immunity were highlighted. In Gene Ontology analysis, the DEGs that participated in immune response, innate immune response and the regulation of apoptotic process were identified as enriched classes. Kyoto Encyclopedia of Genes and Genomes pathway results indicated that most DEGs caused by SCRV infection were identified in the immune system (retinoic acid‐inducible gene‐I‐like receptor/Toll‐like receptor/nucleotide‐binding oligomerization domain‐like receptor/C‐type lectin receptor signalling pathway), cellular processes, cell growth and death (p53 signalling pathway, cellular senescence, apoptosis and phagosome), and metabolism. Quantitative real‐time PCR was used to further verify the expression levels of 15 immune‐related DEGs. The transcriptome database obtained in this study provided further in‐depth insight into the immune response of S. chuatsi against SCRV.

中文翻译：

---

普通鱼Siniperca chuatsi感染Siniperca chuatsi弹状病毒的免疫应答的转录组分析

作为鱼类弹状病毒，Siniperca chuatsi弹状病毒（SCRV）已给man鱼水产养殖业造成了巨大损失。RNA-seq作为一种有效的转录组研究方法，已被广泛用于研究鱼类对病原体的免疫反应。这项研究报告了SCRV感染在0、24和60小时对沙门氏菌的影响在转录组水平上。总共获得了61,527个高质量的单基因，在Sc24和Sc0组，Sc60和Sc0组以及Sc60和Sc24组之间分别标记了3,095、1,854和227个差异表达基因（DEG）。强调了与先天和适应性免疫有关的基因。在基因本体论分析中，参与免疫应答，先天免疫应答和凋亡过程调节的DEG被确定为丰富的类别。京都基因与基因组百科全书》的途径结果表明，大多数由SCRV感染引起的DEGs在免疫系统中得到了识别（视黄酸诱导的基因I样受体/ Toll样受体/核苷酸结合寡聚化域样受体/ C-型凝集素受体信号传导途径），细胞过程，细胞生长和死亡（p53信号通路，细胞衰老，凋亡和吞噬体）和新陈代谢。实时定量PCR用于进一步验证15种免疫相关DEG的表达水平。在这项研究中获得的转录组数据库提供了对小鼠免疫应答的进一步深入了解。S. chuatsi针对SCRV。