The adsorption of DNA on the surface is the basic task of mesoporous silica nanoparticles (MSN) because of its surface charge characteristics. Therefore, without covalent conjugation, the adsorbed DNA can be used directly as the ideal goalkeeper of MSN to encapsulate various cargoes in pores. However, some critical issues regarding the DNA encapsulated cargoes in MSN remain unknown and almost no systematic research is available. This work constructed a simple MSN loading encapsulation and unblocking model with various non-modified DNA, used UV-vis absorption spectrum, circular dichroic spectrum, and mathematical parameters to evaluate critical issues related to this model. The results revealed that the poly-adenine DNA possess the highest affinity with MSN and excellent cargo encapsulation capability, while the poly-thymine DNA possess the highest unblocking capability. Meanwhile, the signal amplification potential of DNA-MSN encapsulation and unblocking system was also confirmed by the unblock coefficient mathematical model. Based on the new insights regarding the biointerface and interaction between DNA and MSN, we finally constructed an DNA-MSN encapsulation and unblocking system induced easy and precise metal ions detection method.

DNA在表面的吸附是介孔二氧化硅纳米颗粒（MSN）的基本任务，因为它具有表面电荷特性。因此，无需共价结合，被吸附的DNA可以直接用作MSN的理想守门员，将各种货物封装在孔中。但是，有关MSN中DNA包裹货物的一些关键问题仍然未知，几乎没有系统的研究可用。这项工作使用各种未修饰的DNA构建了一个简单的MSN加载封装和解封模型，使用了UV-vis吸收光谱，圆二色性光谱和数学参数来评估与该模型相关的关键问题。结果显示，聚腺嘌呤DNA与MSN的亲和力最高，货物封装能力也很强，而聚胸腺嘧啶DNA具有最高的解封闭能力。同时，通过解块系数数学模型也证实了DNA-MSN包封和解块系统的信号放大潜力。基于有关DNA与MSN之间的生物界面和相互作用的新见解，我们最终构建了一种DNA-MSN包封和解封系统诱导的简便而精确的金属离子检测方法。