The Aegle marmelos essential oils (AMEOs) were loaded on surface of the chitosan nanoparticles (CHs NPs), and it performed against carbapenemase producing K. pneumoniae. The physiochemical characterizations of CHs, CHs NPs and AMEOs-CHs NPs were confirmed by FTIR and powder XRD. The morphological observation, size, shape of the SEM and TEM results confirmed that the AMEOs was successfully coated onto the chitosan surface. Further, the excellent minimum inhibitory activity of AMEOs-CHs NPs against CR K. pneumoniae was observed at 40 μg/mL. The increased death cells, morphological damages, and shape modification were confirmed by confocal laser scanning electron microscope, scanning electron microscope and transmission electron microscope respectively. In addition, the invitro anti-carbapenemase activity, and changed color intensity of AMEOs-CHs treated result confirmed that the AMEOs-CHs has carbapenenemase enzyme degradation ability. Importantly, the fragmented DNA and suppressed VIM 1 and IMP 1 genes expression results were clearly indicated that the AMEOs-CHs was very excellent anti-bacterial material. Finally, the molecular docking results were more supported to the invitro evidences, and proved that the AMEOs-CHs as valuable biomaterials owing to their ability to fight against multi drug resistance bacteria.

将Aegle marmelos香精油（AMEOs）负载在壳聚糖纳米颗粒（CHs NPs）的表面上，并针对产生碳青霉烯酶的肺炎克雷伯菌进行了测试。FTIR和粉末XRD证实了CHs，CHs NPs和AMEOs-CHs NPs的理化特性。形态观察，尺寸，SEM和TEM结果的形状证实了AMEO已成功地涂覆在壳聚糖表面上。此外，AMEOs-CHs NPs对CR肺炎克雷伯菌具有极好的最小抑菌活性。观察到浓度为40μg/ mL。共聚焦激光扫描电子显微镜，扫描电子显微镜和透射电子显微镜分别证实了增加的死亡细胞，形态学损伤和形状改变。此外，体外抗碳青霉烯酶的活性，并改变颜色的AMEOs-CHS处理结果强度证实，AMEOs-CHS具有carbapenenemase酶降解的能力。重要的是，片段化的DNA以及受抑制的VIM 1和IMP 1基因的表达结果清楚地表明，AMEOs-CHs是非常出色的抗菌材料。最后，分子对接的结果得到了体外证据的进一步支持，并证明了AMEOs-CHs具有抗多种耐药细菌的能力，因此成为有价值的生物材料。