Tmprss2 is an emerging molecular target which guides cellular infections of SARS-CoV-2, has been earmarked for interventions against the viral pathologies. The study aims to computationally screen and identifies potential miRNAs, following in vitro experimental validation of miRNA-mediated suppression of Tmprss2 for early prevention of COVID-19. Pool of 163 miRNAs, scrutinized for Tmprss2 binding with three miRNA prediction algorithms, ensued 11 common miRNAs. Further, computational negative energies for association, corroborated miRNA-Tmprss2 interactions, whereas three miRNAs (hsa-miR-214, hsa-miR-98 and hsa-miR-32) based on probability scores ≥0.8 and accessibility to Tmprss2 target have been selected in the Sfold tool. Transfection of miRNA(s) in the Caco-2 cells, quantitatively estimated differential expression, confirming silencing of Tmprss2 with maximum gene suppression by hsa-miR-32 employing novel promising role in CoV-2 pathogenesis. The exalted binding of miRNAs to Tmprss2 and suppression of later advocates their utility as molecular tools for prevention of SARS-CoV-2 viral transmission and replication in humans.

Tmprss2 是一种新兴的分子靶标，可指导 SARS-CoV-2 的细胞感染，已被指定用于针对病毒病理学的干预措施。该研究旨在通过体外计算筛选和识别潜在的 miRNAmiRNA 介导的 Tmprss2 抑制对 COVID-19 早期预防的实验验证。163 种 miRNA 的池，使用三种 miRNA 预测算法仔细检查 Tmprss2 结合，随后得到 11 种常见的 miRNA。此外，用于关联的计算负能量证实了 miRNA-Tmprss2 相互作用，而基于概率得分≥0.8 和 Tmprss2 目标的可及性，选择了三种 miRNA（hsa-miR-214、hsa-miR-98 和 hsa-miR-32）在 Sfold 工具中。在 Caco-2 细胞中转染 miRNA，定量估计差异表达，确认 Tmprss2 的沉默与 hsa-miR-32 的最大基因抑制，在 CoV-2 发病机制中发挥新的有希望的作用。