Nucleic acid-based molecular diagnosis has gained special importance for the detection and early diagnosis of genetic diseases as well as for the control of infectious disease outbreaks. The development of systems that allow for the detection and analysis of nucleic acids in a low-cost and easy-to-use way is of great importance. In this context, we present a combination of a nanotechnology-based approach with the already validated dynamic chemical labeling (DCL) technology, capable of reading nucleic acids with single-base resolution. This system allows for the detection of biotinylated molecular products followed by simple detection using a standard flow cytometer, a widely used platform in clinical and molecular laboratories, and therefore, is easy to implement. This proof-of-concept assay has been developed to detect mutations in KRAS codon 12, as these mutations are highly important in cancer development and cancer treatments.

基于核酸的分子诊断对于遗传疾病的检测和早期诊断以及传染病暴发的控制具有特殊的重要性。开发能够以低成本和易于使用的方式检测和分析核酸的系统非常重要。在这种情况下，我们将基于纳米技术的方法与已经验证的动态化学标记 (DCL) 技术相结合，能够以单碱基分辨率读取核酸。该系统允许检测生物素化分子产品，然后使用标准流式细胞仪进行简单检测，这是临床和分子实验室中广泛使用的平台，因此易于实施。这种概念验证分析已开发用于检测 KRAS 密码子 12 中的突变，