The ultra-high-purity separation of ribose from the mixture of monosugars coming from the hydrolysis of defatted microalgal biomass has been a core issue in the areas of microalgae-based ribose production process for pharmaceutical applications. To address such issue, this study was aimed at developing a customized simulated-moving-bed (SMB) process to steadily recover ribose with ultra-high purity from the aforementioned monosugar mixture. First, the intrinsic parameters of the mixture components containing ribose were determined through single-column experiments, which revealed the issue of a low selectivity between ribose and other impurities. On the basis of the determined intrinsic-parameters, the optimal design of the ribose-separation SMB was conducted in such a way that a proper degree of separation margin for maintaining an ultra-high level of ribose purity steadily throughout the SMB operation could be reflected in a quantitative manner while keeping the degree of the adsorbent-bed utilization as high as possible. These considerations were handled by carrying out the optimization runs of maximizing throughput while forcing the purity and yield constraints to be met not only in the normal situation (no deviations in adsorption parameters) but also in the situation where the adsorption parameters of all components would have a given range of fluctuations. The simulation and experimental works for such optimized ribose-SMB confirmed that it could lead to the continuous-mode separation of ribose with the purity of 99.6% without loss, and the degree of its adsorbent-bed utilization could be kept to the highest level when the worst cases of deviations in adsorption parameters would occur.

从脱脂的微藻生物质水解产生的单糖混合物中超高纯度地分离核糖一直是制药业基于微藻的核糖生产工艺领域的核心问题。为了解决这一问题，本研究旨在开发定制的模拟移动床（SMB）工艺，以稳定地从上述单糖混合物中回收具有超高纯度的核糖。首先，通过单柱实验确定了含有核糖的混合物组分的内在参数，这揭示了核糖与其他杂质之间选择性低的问题。根据确定的内在参数，进行核糖分离SMB的最佳设计时，可以定量地反映出在整个SMB操作中稳定地保持核糖纯度超高水平的适当分离度。吸附剂床利用率尽可能高。这些考虑是通过执行最大化产量的优化过程来处理的，不仅要在正常情况下（吸附参数无偏差）而且要在所有组分的吸附参数均具有的情况下满足纯度和收率限制。给定的波动范围。对这种优化的核糖SMB进行的仿真和实验工作证实，它可以导致核糖的连续模式分离，其纯度为99.6％，而不会损失，