Objectives

Anxiety disorder is a common non-motor symptom in patient with Parkinson’s disease (PD). We aimed to explore its pathogenesis and identify plasma biomarkers using untargeted metabolomics analysis.

Methods

Consecutive PD patients and healthy controls were recruited. Clinical data were assessed and patients with Parkinson’s disease related anxiety disorder (PDA) were recognized. Fast plasma samples were obtained and untargeted liquid chromatography-mass spectrometry-based metabolomics analysis was performed. Based on the differentially expressed metabolites from the above metabolomics analysis, correlation analyses and receiver operating characteristic curves (ROC) were further employed.

Results

According to the clinical data, PDA patients had lower plasma levels of total cholesterol, triglyceride, low-density lipoprotein cholesterol, and apolipoprotein B. There were thirty-nine differentially expressed metabolites in PDA patients when compared with the other two groups from the metabolomics analysis, respectively. Fourteen lipid metabolites were simultaneously altered between these two groups, and all of them were significantly decreased. They can be further subcategorized into fatty acyls, glycerolipids, sterol lipids, sphingolipids, and prenol lipids. The plasma levels of thirteen metabolites were negatively correlated with HAMA scores except 10-oxo-nonadecanoic acid. Based on the ROC curves, the fourteen lipid metabolites can be diagnostic biomarkers for PDA patients separately and the areas under the curve of the fourteen lipid metabolites ranged from 0.681 to 0.798.

Conclusions

Significantly lower plasma lipoproteins can be found in PDA patients. A panel of fourteen lipid metabolites were also significantly decreased and can be clinical biomarkers for the diagnosis of PDA patients.

中文翻译：

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代谢组学分析揭示了脂质代谢改变，并将一组脂质代谢物鉴定为帕金森氏病相关焦虑症的生物标志物

目标

焦虑症是帕金森氏病（PD）患者的常见非运动症状。我们旨在探索其发病机理，并使用非靶向代谢组学分析鉴定血浆生物标志物。

方法

连续招募PD患者和健康对照者。评估临床数据并确认患有帕金森氏病相关焦虑症（PDA）的患者。获得快速血浆样品，并进行基于非靶向液相色谱-质谱的代谢组学分析。基于上述代谢组学分析中差异表达的代谢物，进一步采用相关性分析和接收器工作特征曲线（ROC）。

结果

根据临床数据，PDA患者的血浆总胆固醇，甘油三酸酯，低密度脂蛋白胆固醇和载脂蛋白B含量较低。通过代谢组学分析，与其他两组患者相比，PDA患者中有39种差异表达的代谢产物， 分别。两组之间同时改变了14种脂质代谢物，所有这些物质均显着降低。它们可以进一步细分为脂肪酰基，甘油脂，固醇脂，鞘脂和prenol脂。血浆中13种代谢产物的水平与HAMA评分呈负相关，除了10-氧代-壬二酸。根据ROC曲线，

结论

在PDA患者中可发现血浆脂蛋白明显降低。一组十四种脂质代谢物也显着减少，可以作为诊断PDA患者的临床生物标志物。