The Entamoeba histolytica parasite is the causative agent of amebiasis, infecting approximately 1% of the world population and causing 100,000 deaths per year. It binds to Fibronectin (FN), activating signaling pathways regulated by kinases and phosphatases. EhLMW-PTPs genes from E. histolytica encode for Low Molecular Weight Tyrosine Phosphatases expressed in trophozoites and amoebic cysts. The role of these phosphatases in the virulence of the parasite has not yet been well characterized. Our results showed a differential expression of the EhLMW-PTPs, at the mRNA and protein levels, in an asynchronous trophozoites culture. Furthermore, we observed that trophozoites transfected that overexpressed EhLMW-PTP2 phagocytized fewer erythrocytes, possibly due to decreased phagocytic cups, and showed deficiencies in adherence to FN and less cytopathic effect. These analyzes suggest that the parasite's EhLMW-PTPs have an essential role in the mechanisms of proliferation, adhesion, and phagocytosis, regulating its pathogenicity.

该溶组织内阿米巴寄生虫阿米巴病的病原体，感染世界人口的1％左右，并每年导致10名万人死亡。它与纤连蛋白 (FN) 结合，激活由激酶和磷酸酶调节的信号通路。来自溶组织大肠杆菌的EhLMW-PTPs 基因编码在滋养体和阿米巴包囊中表达的低分子量酪氨酸磷酸酶。这些磷酸酶在寄生虫毒力中的作用尚未得到很好的表征。我们的结果显示，在异步滋养体培养中，EhLMW-PTP 在 mRNA 和蛋白质水平上的差异表达。此外，我们观察到转染过表达 EhLMW-PTP2 的滋养体吞噬了较少的红细胞，这可能是由于吞噬杯减少，并且显示出对 FN 的粘附不足和细胞病变效应较小。这些分析表明，寄生虫的 EhLMW-PTPs 在增殖、粘附和吞噬作用的机制中具有重要作用，调节其致病性。