Amyloid fibrils’ stable structure and nice biocompatibility make them good candidates as the templates used in engineering and biomedicine. While palladium nanoparticles have been widely used as catalysts, their high surface energy results in aggregation of nanoparticles, leading to decreased catalytic activities. Here, we investigated the catalytic performance of palladium nanoparticles-deposited lysozyme amyloid fibrils through the reduction of methylene blue (MB). The palladium nanoparticles-decorated lysozyme fibrils were first synthesized and then characterized using several analytical tools. The progress of reductive MB degradation in the presence of sodium borohydride was monitored via UV–Vis spectrophotometry to investigate the catalytic efficiency of bare palladium nanoparticles and palladium nanoparticle/lysozyme fibril hybrids under different conditions. The kinetic data were analyzed using a pseudo-first order model. Finally, the magnitudes of apparent rate constants under different conditions and associated activation parameters were determined. We found that the level of nanoparticle aggregation decreased and the degree of MB conversion significantly increased upon incorporation of amyloid fibrils as the supporting carriers. Moreover, a lower activation energy was perceived when MB reduction was catalyzed by the palladium nanoparticle/fibril hybrids as compared with the bare nanoparticle counterpart. This study presents a nice example of applying amyloid fibril-based hybrid materials in one of the important engineering fields - catalysis.

淀粉样蛋白原纤维的稳定结构和良好的生物相容性使其成为工程和生物医学中的模板。尽管钯纳米颗粒已被广泛用作催化剂，但它们的高表面能导致纳米颗粒聚集，从而导致催化活性下降。在这里，我们研究了通过亚甲基蓝（MB）还原沉积钯纳米粒子的溶菌酶淀粉样原纤维的催化性能。首先合成了装饰有钯纳米粒子的溶菌酶原纤维，然后使用多种分析工具对其进行了表征。通过紫外可见分光光度法监测在硼氢化钠存在下还原性MB降解的进程，以研究裸钯钯纳米粒子和钯纳米粒子/溶菌酶原纤维杂化物在不同条件下的催化效率。使用伪一阶模型分析动力学数据。最后，确定了在不同条件下的表观速率常数的大小以及相关的激活参数。我们发现，当淀粉样蛋白原纤维作为支持载体掺入时，纳米颗粒聚集的水平降低并且MB转化的程度显着增加。此外，与裸纳米粒子相比，当钯纳米粒子/原纤维杂化体催化MB还原时，可以感知到较低的活化能。