Cytolethal distending toxins (CDTs) produced by P. alcalifaciens are considered as potential virulence factors. A loop-mediated isothermal amplification (LAMP) method for the detection of cdtA, cdtB, and cdtC genes was established which showed high specificity and strong sensitivity. The LAMP assay showed a detection threshold was 3.13 pg/μl within 40 min.

中文翻译：

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灵敏、快速检测普罗维登西亚藻中细胞致死膨胀毒素编码基因

P. alcalifaciens产生的细胞致死膨胀毒素 (CDT)被认为是潜在的毒力因子。建立了一种检测cdtA、cdtB和cdtC基因的环介导等温扩增(LAMP)方法，该方法特异性高，灵敏度高。LAMP 检测显示 40 分钟内的检测阈值为 3.13 pg/μl。