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Sensitive and rapid detection of cytolethal distending toxin encoding genes in Providencia alcalifaciens
Journal of Microbiological Methods ( IF 1.7 ) Pub Date : 2021-01-09 , DOI: 10.1016/j.mimet.2021.106143 Xiao Wei 1 , Yan Li 1 , Shan Jiang 1 , Hua Shi 1 , Rongtao Zhao 1 , Zhengquan Yuan 1 , Ying Bai 2 , Yan Li 2 , Qunling Feng 2 , Xiangna Zhao 1
中文翻译:
灵敏、快速检测普罗维登西亚藻中细胞致死膨胀毒素编码基因
更新日期:2021-01-19
Journal of Microbiological Methods ( IF 1.7 ) Pub Date : 2021-01-09 , DOI: 10.1016/j.mimet.2021.106143 Xiao Wei 1 , Yan Li 1 , Shan Jiang 1 , Hua Shi 1 , Rongtao Zhao 1 , Zhengquan Yuan 1 , Ying Bai 2 , Yan Li 2 , Qunling Feng 2 , Xiangna Zhao 1
Affiliation
Cytolethal distending toxins (CDTs) produced by P. alcalifaciens are considered as potential virulence factors. A loop-mediated isothermal amplification (LAMP) method for the detection of cdtA, cdtB, and cdtC genes was established which showed high specificity and strong sensitivity. The LAMP assay showed a detection threshold was 3.13 pg/μl within 40 min.
中文翻译:
灵敏、快速检测普罗维登西亚藻中细胞致死膨胀毒素编码基因
P. alcalifaciens产生的细胞致死膨胀毒素 (CDT)被认为是潜在的毒力因子。建立了一种检测cdtA、cdtB和cdtC基因的环介导等温扩增(LAMP)方法,该方法特异性高,灵敏度高。LAMP 检测显示 40 分钟内的检测阈值为 3.13 pg/μl。