Fenugreek (Trigonella foenum-graecum L.) is a member of the Fabaceae family which is an annual, dicotyledonous and self-pollinated plant. Since genetic diversity is the basis of plant breeding programs, it is important to create genetic mutations in order to increase diversity. To induce mutation, this experiment was conducted by applying four levels (0.1, 0.2, 0.3, and 0.4 %) of ethyl methane sulfonate (EMS) on fenugreek seeds. The treated seeds were planted in the research greenhouse on basis of randomized complete block design with three replications. The results showed that different EMS concentrations had significant effect on all the studied characteristics. The lowest values of the studied traits were observed in the control. Although the highest values of most morpho-physiological traits were obtained in 0.2 % of EMS, but the highest amount of phytochemical compounds were related higher concentrations of EMS. The content of trigonelline, diosgenin, nicotinic acid, and mucilage of grain were significantly increased with increasing EMS concentration. The highest and lowest contents of grains trigonelline were obtained in 0.3 % of EMS and control treatment, respectively. The nicotinic acid content had a positive and significant correlation with trigonelline, diosgenin and mucilage content. Also, the diosgenin content of grains had a positive significant correlation with mucilage content. In general, the application of sufficient concentrations of EMS mutants could be used as an effective tool to increase grain yield and secondary metabolites in breeding programs of fenugreek.

胡芦巴（Trigonella foenum-graecumL.）是Fabaceae家族的成员，该家族是一年生，双子叶和自花授粉的植物。由于遗传多样性是植物育种计划的基础，因此重要的是创建遗传突变以增加多样性。为了诱导突变，通过在胡芦巴种子上施用四种水平（0.1％，0.2％，0.3％和0.4％）的甲烷磺酸乙酯（EMS）来进行该实验。将处理过的种子种植在研究温室中，并根据随机完整块设计进行三次重复。结果表明，不同的EMS浓度对所有研究的特性都有显着影响。在对照中观察到最低的研究性状。尽管在0.2％的EMS中获得了大多数形态生理性状的最高值，但是，最高的植物化学物质含量与较高的EMS浓度有关。随着EMS浓度的增加，藜芦碱，薯os皂苷元，烟酸和谷物的黏液含量显着增加。在0.3％的EMS和对照处理中，分别获得了藜芦碱谷物的最高和最低含量。烟酸含量与三角果碱，薯os皂苷元和黏液含量呈正相关且显着相关。另外，谷物中薯os皂苷元含量与黏液含量呈正相关。通常，在胡芦巴育种程序中，足够浓度的EMS突变体可以用作增加谷物产量和次生代谢产物的有效工具。随着EMS浓度的增加，籽粒的黏度和黏度显着增加。在0.3％的EMS和对照处理中，分别获得了藜芦碱谷物的最高和最低含量。烟酸含量与三角果碱，薯os皂苷元和黏液含量呈正相关且显着相关。另外，谷物中薯os皂苷元含量与黏液含量呈正相关。通常，在胡芦巴育种程序中，足够浓度的EMS突变体可以用作增加谷物产量和次生代谢产物的有效工具。随着EMS浓度的增加，籽粒的黏度和黏度显着增加。在0.3％的EMS和对照处理中，分别获得了藜芦碱谷物的最高和最低含量。烟酸含量与三角果碱，薯os皂苷元和黏液含量呈正相关且显着相关。另外，谷物中薯os皂苷元含量与黏液含量呈正相关。通常，在胡芦巴育种程序中，足够浓度的EMS突变体可以用作增加谷物产量和次生代谢产物的有效工具。烟酸含量与三角果碱，薯os皂苷元和黏液含量呈正相关且显着相关。另外，谷物中薯os皂苷元含量与黏液含量呈正相关。通常，在胡芦巴育种程序中，足够浓度的EMS突变体可以用作增加谷物产量和次生代谢产物的有效工具。烟酸含量与三角果碱，薯os皂苷元和黏液含量呈正相关且显着相关。另外，谷物中薯os皂苷元含量与黏液含量呈正相关。通常，在胡芦巴育种程序中，足够浓度的EMS突变体可以用作增加谷物产量和次生代谢产物的有效工具。