The objective of this study was to assess the long-term effect of peracetic acid (PAA) solution on anoxic organic matter degradation and denitrification. Three bioreactors (R1, R2, and R3) were operated for 166 d, fed with poultry processing wastewater and gradually increased H₂O₂ (R2) or PAA solution (i.e., PAA + H₂O₂)(R3). Organic matter degradation, quantified as the extent of COD removal, and denitrification were assessed. COD removal and denitrification were not affected by H₂O₂ and PAA solutions added to the wastewater before feeding (indirect addition). However, both processes were significantly affected by high levels of H₂O₂ (i.e., 27 mg/L) and PAA solution (i.e., 60/8.4 mg/L PAA/H₂O₂) directly added to the reactors, but fast recovery was observed when direct addition of H₂O₂ and PAA solution ended. The negative impact on denitrification was the combined result of enzyme inhibition and loss of cell viability, both related to increased intracellular ROS levels. Partial acclimation to PAA solution took place during the long-term operation, resulting in higher denitrification activity, higher cell viability and lower intracellular ROS than in a short-term batch assay with one-time PAA addition. Microbiome analysis showed major shift in community composition during the long-term operation with PAA solution addition, but no significant shift after the long-term operation with H₂O₂ addition. Among the main denitrifying genera, Thauera and unclassified Chitinophagaceae were negatively impacted while Chryseobacterium and unclassified Enterobacteriaceae were enriched during the long-term operation with PAA addition.

这项研究的目的是评估过氧乙酸（PAA）溶液对缺氧有机物降解和反硝化的长期影响。三个生物反应器（R1，R2和R3）运行166天，喂入家禽加工废水并逐渐增加H ₂ O ₂（R2）或PAA溶液（即PAA + H ₂ O ₂）（R3）。评估了有机物的降解（量化为COD去除的程度）和反硝化作用。在进料（间接添加）之前向废水中添加H ₂ O ₂和PAA溶液不会影响COD的去除和反硝化。但是，这两个过程都受到高水平H ₂ O _2的显着影响。直接添加到反应器中（即27 mg / L）和PAA溶液（即60 / 8.4 mg / L PAA / H ₂ O ₂），但是当直接添加H ₂ O ₂时观察到快速回收PAA解决方案结束了。对反硝化作用的负面影响是酶抑制和细胞活力丧失的综合结果，两者均与细胞内ROS水平升高有关。在长期操作过程中，部分适应了PAA溶液，与添加了一次PAA的短期分批测定法相比，具有更高的反硝化活性，更高的细胞活力和更低的细胞内ROS。微生物组分析显示，在添加PAA溶液的长期手术过程中，群落组成发生了重大变化，但在添加H ₂ O ₂的长期手术后，群落组成没有明显变化。在主要的反硝化属中，Thauera和未分类的甲壳菌科受到负面影响，而金黄和未分类的肠杆菌科细菌与PAA除了长期运行过程中富集。