In this paper, we developed a novel electrochemiluminescence (ECL) sensing system based on polydopamine nanoparticles (PDA NPs) and MoS₂ nanosheet aerogel. PDA NPs with phenylboronic acid (PBA) were synthesized as a novel kind of organic nano-luminophores. The PDA-PBA NPs had high ECL efficiency and regulable emitting wavelength (552 ~ 610 nm). MoS₂ nanosheet aerogel played the important role in the improvement of ECL sensing performance. On the one hand, MoS₂ nanosheet aerogel can load a large number of PDA-PBA NPs. The accommodation of PDA-PBA NPs in the MoS₂ nanosheet aerogel kept the dispersed state, which prevented NPs aggregation and ECL quenching effect. On the other hand, the excellent conductivity of MoS₂ nanosheet aerogel significantly facilitated the electron transfer. Electron injection with MoS₂ nanosheet aerogel can effectively reduce the damage of PDA-PBA NPs by excessive hot electrons. As a result, the ECL signal of PDA-PBA NPs@MoS₂ nanosheet aerogel have been amplified over 60 times than PDA NPs. Furthermore, the bright ECL light of PDA-PBA NPs@MoS₂ nanosheet aerogel can be clearly observed with the smartphone. Finally, a target-catalyzed hairpin assembly (CHA) sensing mode was designed to visually detect MiRNA-126 (detection limit as 0.56 aM). The MoS₂ nanosheet aerogel-based sensing system provided a new pathway in the development of ECL analysis research.

在本文中，我们开发了一种基于聚多巴胺纳米颗粒（PDA NPs）和MoS ₂纳米片气凝胶的新型电化学发光（ECL）传感系统。合成了具有苯基硼酸（PBA）的PDA NPs，作为一种新型的有机纳米发光体。PDA-PBA NP具有较高的ECL效率和可调节的发射波长（552〜610 nm）。MoS ₂纳米片气凝胶在改善ECL感测性能中起着重要作用。一方面，MoS ₂纳米片气凝胶可以负载大量PDA-PBA NP。PDA-PBA NP在MoS ₂纳米片气凝胶中的保持保持分散状态，从而阻止了NP聚集和ECL猝灭作用。另一方面，MoS ₂的优异电导率纳米片气凝胶显着促进了电子转移。MoS ₂纳米片气凝胶的电子注入可以有效地减少过多的热电子对PDA-PBA NP的破坏。结果，PDA-PBA NPs @ MoS ₂纳米片气凝胶的ECL信号已比PDA NPs放大了60倍以上。此外，使用智能手机可以清晰地观察到PDA-PBA NPs @ MoS ₂纳米片气凝胶的明亮ECL光。最后，设计了一种目标催化的发夹装配（CHA）感应模式，以可视方式检测MiRNA-126（检测极限为0.56 aM）。基于MoS ₂纳米片气凝胶的传感系统为ECL分析研究的发展提供了一条新途径。