Adventitious root (AR) culture is an efficient route for the production of bioactive compounds of Oplopanax elatus. In fed-batch culture, selecting feeding media is a critical strategy for the successful culture. Therefore, this study examined the effects of four feeding medium factors (sucrose concentration, medium salt strength, phosphorus and nitrogen concentration) on culture efficiency of O. elatus ARs by investigating AR dry weight and flavonoid and polysaccharide contents, and the optimal conditions were confirmed by using the analytic hierarchy process (AHP). Results showed that the sucrose concentration affected AR dry weight and polysaccharide content but unaffected flavonoid content. The maximum AR dry weight, flavonoid and polysaccharide contents were obtained with different salt strengths of Murashige and Skoog (MS) medium. Although the phosphorus and nitrogen concentrations did not affect AR dry weight, they affected flavonoid and polysaccharide contents. The optimal conditions of the above four factors, namely, 60 g/L sucrose, 1-fold salt strength of MS medium, 0.94 mM phosphorus, and 30 mM nitrogen, were confirmed on the basis of comprehensive scores from AHP analysis. The findings of the present study provide a reference for further perfectly establishing a fed-batch culture system of O. elatus ARs. The application of AHP method lays the foundation for experiments with multiple indexes to analyze data scientifically and reliably in the field of plant cell culture.

不定根（AR）培养是生产Opopanax elatus生物活性化合物的有效途径。在分批补料培养中，选择补料培养基是成功培养的关键策略。因此，本研究考察了四种饲喂培养基因素（蔗糖浓度，中等盐度，磷和氮浓度）对稻草培养效率的影响。通过研究AR的干重，类黄酮和多糖含量，确定了ARs，并通过层次分析法（AHP）确定了最佳条件。结果表明，蔗糖浓度影响AR干重和多糖含量，但不影响类黄酮含量。在Murashige和Skoog（MS）培养基的不同盐浓度下，获得了最大的AR干重，类黄酮和多糖含量。尽管磷和氮的浓度不影响AR干重，但它们会影响类黄酮和多糖含量。根据AHP分析的综合得分，确定了上述四个因素的最佳条件，即60 g / L蔗糖，MS培养基的1倍盐强度，0.94 mM磷和30 mM氮。O. elatus ARs。层次分析法的应用为多种指标的实验奠定了基础，可以科学，可靠地分析植物细胞培养领域的数据。