A polychaete-assisted sand filter (PASF) can aid water recirculation systems for shrimp culture ponds by removing large amounts of nutrients and suspended solids and chemically treating wastewater. By ingesting and degrading organic matter, Perinereis helleri polychaetes reared in a PASF can accumulate and potentially remove infectious hypodermal and haemopoietic necrosis virus (IHHNV) present in wastewater from ponds rearing virus-infected Penaeus monodon shrimp. Reported here are data showing that filtering pond wastewater through a PASF reduces its potential to transmit IHHNV. The trial employed 3 groups of 4 tanks each containing 20 P. monodon randomly selected from a pond in which IHHNV was evident at moderate prevalence and low loads (IHHNV low-load Pond 2). Over a 2 week period, each tank group was supplied with wastewater from either the same pond (Pond 2) or Pond 1 in which IHHNV was 100% prevalent at ~10⁴-fold higher infection loads, either directly or after being filtered through a PASF. IHHNV real-time qPCR data on total nucleic acid (TNA) extracted from pleopods of 35 P. monodon selected at random from each group identified an elevated IHHNV infection prevalence (91%) in shrimp tested from tanks supplied directly with wastewater from Pond 1. In comparison, IHHNV was detected at a much reduced prevalence and lower loads among shrimp from tanks supplied with wastewater from Pond 2 (33%) or from Pond 1 after it had been filtered through a PASF (31%). The IHHNV prevalence and load data indicate that a PASF can play a useful role in reducing the potential for shrimp pond wastewater to transmit IHHNV infection to naïve P. monodon.

多沙皮辅助砂滤器（PASF）可通过去除大量养分和悬浮固体并对废水进行化学处理，从而有助于虾类养殖池的水循环系统。通过摄食和降解有机物，在PASF中饲养的百里香夜蛾多毛et能够积聚并潜在地清除养殖病毒感染的对虾对虾的池塘中存在的感染性皮下和造血性坏死病毒（IHHNV）。此处报告的数据表明，通过PASF过滤池塘废水会降低其传播IHHNV的潜力。该试验使用3组4个水箱，每个水箱包含20个斑节对虾从中度流行和低负荷时明显出现IHHNV的池塘中随机选择（IHHNV低负荷池塘2）。在2周的时间内，每罐组用从废水或者相同池（池2）或池1，其中IHHNV率为100％流行在〜10供给⁴倍更高感染的负载，直接或通过一个过滤后PASF。从35个斑节对虾的足节中提取的总核酸（TNA）的IHHNV实时qPCR数据从各组中随机选择，发现直接从池塘1的污水供应的水箱中检测的虾中IHHNV感染率升高（91％）。相比之下，从装有污水的水箱中的虾中检测到IHHNV的流行率大大降低且负荷较低从池塘2（33％）或池塘1中通过PASF过滤后（31％）。IHHNV的患病率和负荷数据表明，PASF可以在减少虾塘废水将IHHNV感染传播给幼稚的斑节对虾中发挥潜在作用。